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Related Experiment Videos

Pig mitochondrial DNA: polymorphism, restriction map orientation, and sequence data.

T Watanabe, Y Hayashi, J Kimura

    Biochemical Genetics
    |June 1, 1986
    PubMed
    Summary

    Pig mitochondrial DNA (mtDNA) analysis reveals polymorphisms, supporting dual maternal origins from European and Asian wild boars. This genetic diversity explains the ancestry of breeds like the Large White.

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Zoology

    Background:

    • Mitochondrial DNA (mtDNA) analysis is crucial for understanding animal origins and population genetics.
    • Previous studies suggested pigs may have diverse maternal lineages.

    Purpose of the Study:

    • To analyze restriction endonuclease cleavage patterns of pig mitochondrial DNA (mtDNA).
    • To investigate mtDNA polymorphisms in Taiwan native breeds and Japanese miniature strains.
    • To refine the restriction endonuclease cleavage map of pig mtDNA and compare it with other mammalian species.

    Main Methods:

    • Restriction endonuclease digestion of pig mtDNA using 18 six-nucleotide and 1 four-nucleotide recognizing enzymes.
    • Cloning of HindIII cleavage fragments into M13mp10 for partial sequencing.

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  • Dideoxynucleotide-chain termination sequencing method.
  • Comparative sequence analysis of pig mtDNA with cow, human, mouse, and rat mtDNA.

    • Main Results:

    • Polymorphisms were observed in cleavage patterns with five restriction enzymes (Bg1II, EcoRV, ScaI, StuI, TaqI).
    • Results support the hypothesis of two maternal origins for pigs: European and Asian wild boars.
    • The Large White breed shows evidence of both European and Asian ancestry.
    • Newly determined DraI and StuI cleavage sites refined the pig mtDNA restriction map.
    • Sequence comparisons revealed evolutionary relationships and genetic differences among mammalian mtDNAs.

    Conclusions:

    • Pig mtDNA polymorphisms confirm dual maternal origins, contributing to breed diversity.
    • The study provides an updated restriction map of pig mtDNA.
    • Comparative sequence analysis elucidates evolutionary divergence among mammalian species.