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Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella

Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella

Published on: May 13, 2019

Smartphone-based mobile digital PCR device for DNA quantitative analysis with high accuracy.

Tong Gou¹, Jiumei Hu², Wenshuai Wu²

¹Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310027, PR China.

Biosensors & Bioelectronics

|September 3, 2018

Summary

A new smartphone-based digital polymerase chain reaction (dPCR) device offers accurate nucleic acid quantification. This portable, low-cost tool is ideal for point-of-care applications in resource-limited settings.

Keywords:

Cancer biomarker gene Digital PCR Microfluidic chip Point-of-care Resource-limited settings Smartphone

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Area of Science:

Biotechnology
Molecular Biology
Analytical Chemistry

Background:

Digital polymerase chain reaction (dPCR) offers absolute nucleic acid quantification but existing devices lack portability and affordability.
Limited infrastructure in certain laboratories or regions restricts access to advanced dPCR technology.

Purpose of the Study:

To develop a mobile, integrated dPCR device suitable for resource-limited environments.
To enable accurate DNA quantitative analysis outside of traditional laboratory settings.

Main Methods:

Development of a smartphone-based dPCR device integrating thermal cycling, on-chip dPCR, data acquisition, and analysis.
Utilized a customized Android software for automated control of all device functions.
Employed a self-priming dPCR chip for nucleic acid quantification.

Main Results:

The developed dPCR device is compact (90x90x100 mm, 500g) and cost-effective (~$320 excluding smartphone).
Achieved accurate quantification down to 10 copies of human 18S ribosomal DNA, comparable to commercial platforms.
Demonstrated capability to detect single molecules of the cancer biomarker gene CD147 in HepG2 cells.

Conclusions:

The developed smartphone-based dPCR device is a low-cost, portable, and robust tool for accurate DNA quantification.
This technology holds significant potential for point-of-care (POC) applications, especially in settings with limited infrastructure.