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Updated: Feb 5, 2026

Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM
Published on: March 2, 2017
The determining role of nanoscale mechanical twinning on cellular functions of nanostructured materials
K C Nune1, I Montes1, V S Y Injeti1
1Department of Metallurgical, Materials and Biomedical Engineering, University of Texas at El Paso, 500 W. University Avenue, El Paso, TX 79968, USA.
Abstract:
Considering that micromotions generated at the bone-implant interface under physiological loading introduce mechanical strain on the tissue and surface of the implant and that strain can be introduced during processing of the biomedical device, we elucidate here the interplay between mechanically-induced nanoscale twinning in austenitic stainless steel on osteoblast functions. Mechanically-induced nanoscale twinning significantly impacted cell attachment, cell-substrate interactions, proliferation, and subsequent synthesis of prominent proteins (fibronectin, actin, and vinculin). Twinning was beneficial in favorably modulating cellular activity and contributed to small differences in hydrophilicity and nanoscale roughness in relation to the untwinned surface.
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