Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confirmation Biases01:31

Confirmation Biases

8.3K
The confirmation bias is the tendency to focus on information that confirms our existing beliefs and ignore information that is inconsistent with our expectations. For example, if you think that your professor is not very nice, you notice all of the instances of rude behavior exhibited by the professor while ignoring the countless pleasant interactions he is involved in on a daily basis. Have you ever fallen prey to the confirmation bias, either as the source or target of such bias?
8.3K
Hindsight Biases01:12

Hindsight Biases

4.3K
Hindsight bias leads you to believe that the event you just experienced was predictable, even though it really wasn’t. In other words, you knew all along that things would turn out the way they did. Can you relate this to the phrase "Hindsight is 20/20" now? 
4.3K
Decreasing Function01:27

Decreasing Function

299
A decreasing function describes a relationship where the output consistently declines as the input increases. This means that for any two input values, if one is greater than the other, the corresponding output is smaller. Mathematically, a function f is decreasing on an interval I if for every x1 < x2​ in I, f (x1) > f (x2). This type of behavior is visually identified on a graph that slopes downward from left to right.The nature of a function can be analyzed by calculating...
299
Bias01:22

Bias

7.4K
Bias refers to any tendency that prevents a question from being considered unprejudiced. In research, bias occurs when one outcome or answer is selected or encouraged over others in sampling or testing. Bias can occur during any research phase, including study design, data collection, analysis, and publication.
In statistics, a sampling bias is created when a sample is collected from a population, and some members of the population are not as likely to be chosen as others (remember, each member...
7.4K
Decreased Body Temperature01:29

Decreased Body Temperature

1.1K
A decreased body temperature can occur in patients with hypothermia and frostbite. Heat loss with extended cold exposure overpowers the body's ability to create heat, resulting in hypothermia. Core temperature readings help classify hypothermia. Mild hypothermia is temperatures between 32 °C (89.6 °F) and 35°C (95 °F) and is caused by impaired thermoregulation. Moderate hypothermia is temperatures between 28 C (82.4 °F) and 32 °C (89.6 °F) caused by...
1.1K
Decreased pulse rate01:14

Decreased pulse rate

905
Bradycardia is a medical condition in which the heart rate is slower than normal. It occurs when the heart's natural pacemaker, the sinus node, generates slower electrical impulses than the standard rhythm. In adults, bradycardia is diagnosed when the pulse rate falls below 60 beats per minute, indicating a deviation from the normal heart rate range.
There are specific risk factors that can elevate the likelihood of developing bradycardia. Advanced age is a significant factor, with...
905

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptomic characterization of Coccidioides morphological states using RiboMarker-enhanced RNA sequencing.

G3 (Bethesda, Md.)·2026
Same author

A method of comprehensive sequencing analysis of the small RNA fragmentome (RiboMarker).

RNA (New York, N.Y.)·2026
Same author

A method of comprehensive sequencing analysis of the small RNA fragmentome (RiboMarker <sup>®</sup> ).

bioRxiv : the preprint server for biology·2025
Same author

Multiomics Profiling of Extracellular Vesicles Supports Their Involvement in Endothelial Senescence-Associated Vascular Dysfunction.

Journal of extracellular biology·2025
Same author

Spatiotemporal perturbations of the plasminogen activation system in a rat model of acute organophosphate intoxication.

Acta neuropathologica communications·2025
Same author

Characterization of transcriptomic changes across <i>Coccidioides</i> morphologies using RiboMarker®-enhanced RNA sequencing.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Feb 5, 2026

Detection of Copy Number Alterations Using Single Cell Sequencing
09:45

Detection of Copy Number Alterations Using Single Cell Sequencing

Published on: February 17, 2017

12.2K

Decreasing miRNA sequencing bias using a single adapter and circularization approach.

Sergio Barberán-Soler1, Jenny M Vo2, Ryan E Hogans2

  • 1SomaGenics, Inc., Santa Cruz, California, USA. sbarberan@somagenics.com.

Genome Biology
|September 4, 2018
PubMed
Summary

Accurately quantifying microRNAs (miRNAs) is crucial for biology and therapeutics. A new method, RealSeq®-AC, reduces sequencing bias, enabling broader miRNA identification and robust quantification across diverse RNA inputs.

Keywords:
Sequencing biasSmall RNA library preparationSmall RNA sequencingmiRNA-seq

More Related Videos

Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.8K
mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

3.0K

Related Experiment Videos

Last Updated: Feb 5, 2026

Detection of Copy Number Alterations Using Single Cell Sequencing
09:45

Detection of Copy Number Alterations Using Single Cell Sequencing

Published on: February 17, 2017

12.2K
Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.8K
mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

3.0K

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Accurate microRNA (miRNA) quantification is vital for understanding miRNA biology.
  • Development of novel biomarkers and therapeutic targets relies on precise miRNA measurement.
  • Existing methods for small RNA sequencing often exhibit bias, limiting comprehensive miRNA profiling.

Purpose of the Study:

  • To develop an improved method for preparing miRNA sequencing libraries.
  • To reduce bias in miRNA sequencing and enhance the diversity of identified miRNAs.
  • To enable robust quantification of miRNAs across a wide range of RNA input levels.

Main Methods:

  • Development of the RealSeq®-AC library preparation method.
  • Ligation of miRNAs with a single adapter.
  • Circularization of ligation products.

Main Results:

  • RealSeq®-AC significantly reduces miRNA sequencing bias compared to other methods.
  • The method allows for the identification of a larger variety of miRNAs in biological samples.
  • Robust quantification of miRNAs is achieved across a broad spectrum of RNA input quantities.

Conclusions:

  • RealSeq®-AC is a superior method for miRNA library preparation.
  • This technique enhances the accuracy and comprehensiveness of miRNA profiling.
  • The method has significant implications for biomarker discovery and therapeutic development.