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Updated: Feb 5, 2026

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira
Published on: August 14, 2021
Heterologous protection elicited by a live, attenuated, Leptospira vaccine
Gerald L Murray1, Theeraya Simawaranon2, Chamraj Kaewraemruaen3
1Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, Victoria 3800, Australia.
Abstract:
A previously-described live, attenuated vaccine (M1352, serovar Manilae, serogroup Pyrogenes) was tested in the hamster model of infection for cross-protective immunity. The vaccine elicited strong, significant cross-protection against lethal infection by strains representing four serologically distinct leptospiral serovars (Grippotyphosa, Australis, Canicola, and Autumnalis). Combined with our previously reported protection against serovars Pomona and Manilae, this work demonstrates unequivocal proof of concept for cross-protective immunity in leptospirosis.
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