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Commodity Chemicals From Engineered Modular Type I Polyketide Synthases.

Satoshi Yuzawa1, Amin Zargar2, Bo Pang3

  • 1Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States; Joint BioEnergy Institute, Emeryville, CA, United States.

Methods in Enzymology
|September 4, 2018
PubMed
Summary
This summary is machine-generated.

Engineered modular polyketide synthases (PKSs) can create novel compounds. This study details methods for PKS engineering to produce valuable commodity chemicals with predictable structures.

Keywords:
Commodity chemicalsMetabolic engineeringPolyketide synthasesProtein engineeringSynthetic biology

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Area of Science:

  • Biotechnology
  • Synthetic Biology
  • Natural Product Synthesis

Background:

  • Reduced polyketides are structurally diverse natural products with broad applications.
  • Modular polyketide synthases (PKSs) are enzyme complexes responsible for their biosynthesis.
  • Understanding the PKS sequence-structure relationship enables targeted engineering.

Purpose of the Study:

  • To describe experimental protocols for engineering modular PKSs.
  • To demonstrate the production of commodity chemicals using engineered PKSs.
  • To enable the creation of novel compounds with predictable structures.

Main Methods:

  • Engineering modular polyketide synthases (PKSs).
  • Developing experimental protocols for PKS domain/module manipulation.
  • Case studies involving PKS engineering for chemical production.

Main Results:

  • Established protocols for modular PKS engineering.
  • Successfully produced commodity chemicals via engineered PKSs.
  • Demonstrated the predictability of compound structures from PKS engineering.

Conclusions:

  • Modular PKSs are highly adaptable targets for engineering.
  • PKS engineering offers a viable route to novel and commodity chemicals.
  • This work provides a framework for future PKS-based biosynthesis endeavors.