Commodity Chemicals From Engineered Modular Type I Polyketide Synthases
Related Experiment Video
Updated: Feb 5, 2026

Standardized Modular Assembly of Polycistronic Operons with Modular Cloning (MoClo) using the In-Cloning toolkit
Published on: September 2, 2025
Satoshi Yuzawa1, Amin Zargar2, Bo Pang3
1Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States; Joint BioEnergy Institute, Emeryville, CA, United States.
Abstract:
Reduced polyketides are a subclass of natural products that have a variety of medical, veterinary, and agricultural applications and are well known for their structural diversity. Although these compounds do not resemble each other, they are all made by a class of enzymes known as modular polyketide synthases (PKSs). The commonality of PKS domains/modules that compose PKSs and the understanding of the relationship between the sequence of the PKS and the structure of the compound it produces render modular PKSs as excellent targets for engineering to produce novel compounds with predicted structures. Here, we describe experimental protocols and considerations for modular PKS engineering and two case studies to produce commodity chemicals by engineered PKSs.
More Related Videos
09:31PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
Published on: September 26, 2020
10:55Construction of Modular Hydrogel Sheets for Micropatterned Macro-scaled 3D Cellular Architecture
Published on: January 11, 2016
Related Concept Videos
Types of Chemical Bonds
Types of Chemical Bonds
Types of Chemical Reactions: Anabolic and Catabolic
Anabolism is the process of combining smaller, simpler molecules into larger, more...
Types of Chemical Reactions: Exchange and Reversible
A special kind of exchange reaction is the oxidation-reduction reaction, or the redox reaction. These reactions involve the transfer of electrons from one compound to another. The electrons in these reactions commonly come from hydrogen atoms, which consist of an electron and a proton. A molecule gives up a...
ATP Synthase: Structure
ATP Synthase: Mechanism