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Updated: Feb 5, 2026

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
Published on: April 23, 2012
Development and validation of a multianalyte method for quantification of mycotoxins and pesticides in rice using a
Lucas Pinto da Silva1, Fernando Madureira2, Eugenia de Azevedo Vargas2
1Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil.
Abstract:
In the present manuscript an analytical methodology for the simultaneous determination of ten mycotoxins and six pesticides in rice was developed. This methodology comprises the application of the dilute and shoot protocol followed by quantification via UHPLC-MS/MS. The methodology was validated and all figures of merit shown to be within the limits established by regulation. Hence, the recoveries for mycotoxins and pesticides were within the specified ranges. Precision was assessed by repeatability and intra-laboratory reproducibility with standard deviations smaller than or equal to 20%. The limits of detection, quantification and decision as well as the detection capacity were determined by the analytical curves whereas the measurement uncertainty was established by applying the bottom-up approach. Finally, the current methodology was applied to samples of rice (n = 42) commercialized in Brazil and positive results were found in only two for deoxynivalenol and zearalenone.
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