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Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model
09:29

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Published on: March 20, 2020

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Identification of Key Genes and Pathways in Triple-Negative Breast Cancer by Integrated Bioinformatics Analysis

Pengzhi Dong1, Bing Yu2, Lanlan Pan1

  • 1Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.

Biomed Research International
|September 4, 2018
PubMed
Abstract

Insights

Researchers identified key genes and pathways involved in triple-negative breast cancer (TNBC) development. Specific genes like SOX8 and AR were linked to patient survival, offering potential new TNBC treatment targets.

Area of Science:

  • Genomics
  • Cancer Biology
  • Bioinformatics

Background:

  • Triple-negative breast cancer (TNBC) lacks expression of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (Her2).
  • Understanding TNBC's initiation and progression mechanisms is crucial for developing effective treatments.

Purpose of the Study:

  • To identify key pathways and genes implicated in TNBC.
  • To elucidate the potential mechanisms driving TNBC initiation and progression.

Main Methods:

  • Gene expression profiles from 265 samples (67 non-TNBC, 198 TNBC) were analyzed from the GSE76275 dataset.
  • Differentially expressed genes (DEGs) were identified using stringent statistical criteria (p<0.01, fold change ≥1.5 or ≤-1.5).
  • Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed on DEGs.

Main Results:

  • 56 genes were upregulated and 151 downregulated in TNBC.
  • DEGs were significantly enriched in pathways related to morphogenesis, cell maturation, nervous system development, and immune processes like T cell differentiation and macrophage activation.
  • Survival analysis revealed that amplification of SOX8, AR, and C9orf152 correlated with poorer outcomes, while NRK and RAB30 showed positive correlation with life expectancy.

Conclusions:

  • The identified pathways and genes provide insights into TNBC development mechanisms.
  • SOX8, AR, C9orf152, NRK, and RAB30 represent potential therapeutic targets for TNBC treatment.

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