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Visualizing and Quantifying the Effect of the Inhibition of HSP70 on Breast Cancer Cells Based on Laser Scanning

Biying Yu1, Hongqin Yang1, Xiaoman Zhang1

  • 11 Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, China.

Technology in Cancer Research & Treatment
|September 4, 2018
PubMed

Insights

Heat shock protein 70 (HSP70) inhibition with VER-155008 induced apoptosis in breast cancer cells. Heat shock partially alleviated VER-155008

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Heat shock protein 70 (HSP70) is overexpressed in breast cancer, correlating with poor prognosis and drug resistance.
  • HSP70 plays a role in cell proliferation and survival.
  • Targeting HSP70 presents a potential therapeutic strategy for breast cancer.

Purpose of the Study:

  • To investigate the effects of HSP70 inhibitor VER-155008 and heat shock on MCF-7 breast cancer cell mitochondria.
  • To evaluate the combined effects of VER-155008 and heat shock on breast cancer cells.
  • To determine the therapeutic potential of targeting HSP70 in breast cancer.

Main Methods:

  • MCF-7 breast cancer cells were treated with VER-155008, heat shock (43°C, 1 hour), or a combination.
  • Mitochondrial integrity and membrane potential were assessed using laser scanning microscopy and fluorescence probes.
  • Cell size and mitochondrial content were quantified.

Main Results:

  • VER-155008 treatment and combination treatment disrupted mitochondrial networks and decreased membrane potential and cell size.
  • Mitochondrial content was reduced by VER-155008 and combination treatments in a time-dependent manner.
  • Heat shock partially alleviated the inhibitory effects of VER-155008, while sole heat shock had no significant effect.

Conclusions:

  • VER-155008 induces apoptosis in MCF-7 breast cancer cells, both alone and in combination with heat shock.
  • Heat shock can modulate the efficacy of HSP70 inhibition, suggesting complex interactions.
  • HSP70 is a viable therapeutic target for breast cancer treatment.
Keywords:
VER-155008breast cancer cellcell apoptosisheat shockheat shock protein 70mitochondrial membrane potential

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