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Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin.

Dechun Zhang1, Xianzhao Kan2, Sarah Elizabeth Huss3

  • 1Key Laboratory of Three Gorges Regional Plant Genetics and Germplasm Enhancement (CTGU)/Biotechnology Research Center, China Three Gorges University.

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|September 4, 2018
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Summary

Phylogenetic analysis reveals the evolutionary origins of eukaryotic Sugars Will Eventually be Exported Transporters (SWEET) genes. The study suggests these genes arose from a fusion of bacterial and archaeal SemiSWEET genes.

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Area of Science:

  • Molecular Biology
  • Evolutionary Biology
  • Bioinformatics

Background:

  • Phylogenetic analysis reconstructs evolutionary relationships using molecular data.
  • It is crucial for understanding taxa with limited fossil records.
  • Investigating gene origins often requires advanced computational methods.

Purpose of the Study:

  • To detail a protocol for phylogenetic analysis of gene families.
  • To investigate the evolutionary origin of eukaryotic SWEET genes.
  • To explore the relationship between prokaryotic SemiSWEET and eukaryotic SWEET proteins.

Main Methods:

  • Amino acid sequence alignment using Clustal Omega.
  • Phylogenetic tree construction via Maximum Likelihood (ML) in MEGA.
  • Bayesian Inference using MrBayes for tree building.

Main Results:

  • Analyzed 228 SWEETs from eukaryotes and 57 SemiSWEETs from prokaryotes.
  • Identified SemiSWEET proteins in prokaryotes and SWEET proteins in eukaryotes.
  • Two distinct phylogenetic methods consistently indicated a fusion origin for eukaryotic SWEET genes from bacterial and archaeal SemiSWEET genes.

Conclusions:

  • Eukaryotic SWEET genes likely originated from the fusion of two prokaryotic SemiSWEET genes.
  • Phylogenetic analysis provides insights into evolutionary relationships difficult to ascertain experimentally.
  • Caution is advised when interpreting phylogenetic results solely.