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Related Experiment Video

Updated: Feb 5, 2026

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
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Full-length RNA sequencing reveals unique transcriptome composition in bermudagrass.

Bing Zhang1, Jianxiu Liu2, Xiaoshan Wang1

  • 1College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.

Plant Physiology and Biochemistry : PPB
|September 4, 2018
PubMed
Summary

This study presents the first full-length transcriptome dataset for bermudagrass (Cynodon dactylon), a valuable turfgrass. The findings reveal enriched C4 photosynthesis genes, explaining its warm-climate adaptation and aiding future breeding.

Keywords:
Alternative isoformBermudagrassC(4) photosynthesisPacBio sequencingTranscriptome

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Area of Science:

  • Plant genomics
  • Transcriptomics
  • Turfgrass science

Background:

  • Bermudagrass (Cynodon dactylon) is an economically important turfgrass.
  • Limited genomic and transcriptomic data hinder functional and breeding studies.

Purpose of the Study:

  • To generate the first comprehensive full-length transcriptome dataset for bermudagrass.
  • To provide insights into gene characteristics and phylogeny.
  • To investigate the genetic basis of warm-climate adaptation.

Main Methods:

  • Single-molecule long-read sequencing (PacBio) was used.
  • A mixed sample of bermudagrass tissues (leaves, stolons, shoots, roots, flowers) was analyzed.
  • Transcriptome data were compared with nine other grass species.

Main Results:

  • A dataset of 78,192 unigenes was generated, with 66,409 functionally annotated.
  • 27,946 unigenes exhibited two or more isoforms.
  • KEGG pathway analysis showed enrichment of C4 photosynthesis-related genes, including phosphoenolpyruvate carboxylase and pyruvate, phosphate dikinase.

Conclusions:

  • The generated transcriptome provides a valuable resource for bermudagrass research.
  • Enriched C4 photosynthesis genes likely contribute to bermudagrass's success in warm environments.
  • This study lays a foundation for future functional genomics and molecular breeding in bermudagrass.