Analysis of Interactions Between Components of Selected Solution for Organ Perfusion and Preservation

In the literature there is a great deal of information about the activity of liquid preservatives and the solutions used to modify them; however, there is no information about the possibility of interactions between them during multiorgan retrieval. The aim of the study was to analyze the possibility of reactions between the components of Biolasol and histidine-tryptophan-ketoglutarate (HTK). Biolasol is the first Polish liquid preservative intended for rinsing kidneys, livers, and pancreases by simple hypothermia. HTK is a cardioplegic fluid used in organ preservation of hearts, livers, kidneys, pancreases, and lungs.

Biolasol and HTK solution were used for the tests. The preservatives were mixed together at 1:1, 0.25:0.75, and 0.75:0.25 ratios. The volume of mixtures to be analyzed was 500 mL. The prepared systems were stored in a refrigerator (4°C) protecting against light. The systems were subjected to physicochemical analysis involving pH, density, viscosity, buffer capacity, osmolarity, and absorbance measurements as well as visual and microscopic assessment at time intervals: immediately after mixing (t = 0) and after 12, 24, 48, and 72 hours.

The analysis suggests that interactions between solution components can occur and have a positive effect on storage time and effectiveness of organ rinsing.

The use of Biolasol and HTK in multiorgan retrieval is safe.