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Updated: Feb 5, 2026

Formulation and Characterization of Bioactive Agent Containing Nanodisks
Published on: March 17, 2023
Structural characterization of a bioactive water-soluble heteropolysaccharide from Nostoc sphaeroids kütz
Yanfang Liu1, Panfeng Su2, Jianfeng Xu3
1Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, National R&D Center for Edible Fungi Processing, Shanghai 201403, China.
Abstract:
A water soluble Nostoc sphaeroids kütz polysaccharide (NSKP) was extracted and purified by precipitating hot-water extract from Nostoc sphaeroids kütz with 40% (v/v) ethanol. The yield was 40.10% (w/w), and weight average molecular weight (Mw) was determined as 1.31 × 105 g/mol. Cell culture study showed that NSKP could stimulate the NO release from RAW264.7 macrophages. The ρ value (Rg/Rh) of NSKP was calculated as 1.50, indicating flexible random coils conformation of NSKP in aqueous solution. According to methylation analysis, and 1D & 2D NMR spectra, the molecular structure of polysaccharide NSKP was deduced as below.
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