Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Converting Sendai virus into a specific fusogen whose cell target can be selected.

O Martinez, J Kimura, C Henry

    Experimental Cell Research
    |September 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Goat and cow-milk based infant formulas contain extracellular vesicles with different miRNA and protein cargo.

    Current research in food science·2026
    Same author

    Financial Toxicity in Cervical Cancer Patients Undergoing Radical Treatment With External Beam Radiation and Brachytherapy at a Tertiary New Zealand Radiation Oncology Centre.

    Journal of medical imaging and radiation oncology·2026
    Same author

    Evaluation of the reproducibility and factors affecting perfusion measurement in normal pregnancies with single-slice FAIR Arterial Spin Labeling (ASL).

    Placenta·2026
    Same author

    Characterization of extracellular vesicles at parturition in dairy cows with late-gestation heat stress.

    JDS communications·2026
    Same author

    Family History and Solar Insolation in Bipolar I Disorder.

    Acta psychiatrica Scandinavica·2026
    Same author

    Chronic meningoencephalitis due to enterovirus A71 complicating rituximab therapy.

    Revue neurologique·2025

    Researchers created antibody-avidin hybrids to redirect Sendai virus (SV) fusion activity. This targeted delivery system harnesses viral fusion for precise macromolecule delivery into specific cells.

    Area of Science:

    • Virology
    • Immunology
    • Bioconjugation

    Background:

    • Sendai virus (SV) possesses hemagglutinin-neuraminidase (HN) glycoproteins crucial for cell fusion and viral entry.
    • Monoclonal antibodies targeting HN can block viral agglutination and fusion.
    • Avidin-biotin interactions offer a versatile platform for molecular conjugation.

    Purpose of the Study:

    • To develop covalent hybrids of Fab anti-HN monoclonal antibody and avidin.
    • To investigate the ability of these conjugates to block and redirect SV fusion activity.
    • To assess the potential of this approach for targeted delivery applications.

    Main Methods:

    • Preparation and characterization of Fab anti-HN: avidin covalent conjugates.
    • Incubation of SV with the Fab anti-HN: avidin conjugate.

    Related Experiment Videos

  • Assessing cell-cell fusion mediated by modified SV with biotin-labeled target cells.

    • Main Results:

    • Fab anti-HN: avidin conjugates successfully blocked normal SV agglutination.
    • Modified SV fused only biotin-labeled target cells, demonstrating redirected specificity.
    • Achieved cell-cell fusion levels were comparable to those with unmodified SV.

    Conclusions:

    • It is feasible to block the natural cell recognition of SV's HN molecules.
    • A new, specific cell recognition feature can be introduced without compromising viral fusogenicity.
    • This strategy enables harnessing SV fusion for targeted delivery of macromolecules into selected cells.