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Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
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Membrane remodeling in clathrin-mediated endocytosis
Volker Haucke1,2, Michael M Kozlov3
1Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Robert-Rössle-Straße 10, 13125 Berlin, Germany haucke@fmp-berlin.de michk@post.tau.ac.il.
Journal of Cell Science
|September 5, 2018
Summary
Clathrin-mediated endocytosis uses protein and lipid interactions to remodel cell membranes. This process is crucial for cellular functions like signaling and migration.
Area of Science:
- Cell Biology
- Biophysics
Background:
- Clathrin-mediated endocytosis (CME) is vital for eukaryotic cells, regulating plasma membrane composition for essential processes.
- CME involves complex protein-mediated membrane remodeling, orchestrated by spatiotemporal protein-protein and protein-lipid interactions.
Purpose of the Study:
- To review recent findings on the temporal and spatial organization of endocytic protein choreography.
- To discuss models explaining how endocytic proteins and lipids reshape the plasma membrane during CME.
Main Methods:
- High-resolution imaging
- Genetic manipulation of the endocytic machinery
- Theoretical approaches
Main Results:
- Novel data on the spatiotemporal organization of the endocytic reaction.
- Conflicting models exist regarding the roles of proteins and lipids in membrane remodeling.
Conclusions:
- Endocytic membrane remodeling is hypothesized to result from an interplay between protein-protein interactions, membrane curvature sensing/generation by proteins, and lipid-induced stabilization of membrane shape.
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