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In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window
Published on: August 4, 2022
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Super-wide-field two-photon imaging with a micro-optical device moving in post-objective space.
Shin-Ichiro Terada1,2,3, Kenta Kobayashi4, Masamichi Ohkura5
1Department of Physiology, Graduate School of Medicine, The University of Tokyo, Tokyo, 113-0033, Japan. sterada@m.u-tokyo.ac.jp.
Nature Communications
|September 5, 2018
Summary
Scientists developed a novel micro-opto-mechanical device for wide-field two-photon microscopy. This technology allows simultaneous imaging of distant brain regions, revealing neural correlations in mouse forelimb areas during movement tasks.
Area of Science:
- Neuroscience
- Optical imaging
- Microscopy
Background:
- Cellular-resolution wide-field neural imaging is challenging.
- Existing wide-field two-photon microscopy is limited by objective size.
Purpose of the Study:
- To develop a novel device to overcome field size limitations in two-photon microscopy.
- To enable imaging of neural activity across distant brain areas.
Main Methods:
- Development of a micro-opto-mechanical device for post-objective rotation.
- Application of the device with two-photon microscopy for sequential calcium imaging.
- Imaging of mouse sensory forelimb areas separated by 6 mm.
Main Results:
- Sub-second sequential calcium imaging of distant mouse forelimb areas (RFA and CFA, 2 mm apart).
- High pairwise correlations observed between spontaneous neural activity in RFA and CFA.
- Population activity between RFA and CFA covaried during a sound-triggered movement task.
Conclusions:
- The micro-opto-mechanical device enables flexible wide-field imaging across large brain distances.
- This technology clarifies correlation structures between distant brain areas at cellular and population levels.
- Provides new insights into neural circuit dynamics and inter-area communication.
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