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Updated: Aug 10, 2026

Robust 3D DNA FISH Using Directly Labeled Probes
Published on: August 15, 2013
A rapid and simple procedure for the preparation of homogeneously labeled DNA probes
Abstract:
This report describes a novel technique for the preparation of homogeneously labeled DNA probes. The procedure uses a heteroduplex intermediate, which is directly prepared from a double-stranded plasmid, and therefore requires neither sub-cloning of the fragment of interest into a specialized vector nor the use of a synthetic primer. The technical advantages of the procedure are demonstrated by preparation of a probe able to determine the precise location and efficiency of utilization of the polyadenylation site used during transcription of the rat preproinsulin II gene in a transfected avian cell line.
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