Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase II Reactions: Methylation Reactions01:17

Phase II Reactions: Methylation Reactions

740
Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...
740
Multiple Halogenation of Methyl Ketones: Haloform Reaction01:28

Multiple Halogenation of Methyl Ketones: Haloform Reaction

2.9K
A method involving the transformation of methyl ketones to carboxylic acids using excess base and halogen is called the haloform reaction. It begins with the deprotonation of α hydrogen to form an enolate ion which reacts with the electrophilic halogen to give an α-halo ketone. The step continues until all the α protons are substituted to form a trihalomethyl ketone. The resulting molecule is unstable, and in the presence of a hydroxide base, it readily undergoes nucleophilic...
2.9K
Qualitative Analysis03:46

Qualitative Analysis

24.5K
For solutions containing mixtures of different cations, the identity of each cation can be determined by qualitative analysis. This technique involves a series of selective precipitations with different chemical reagents, each reaction producing a characteristic precipitate for a specific group of cations. Metal ions within a group are further separated by varying the pH, heating the mixture to redissolve a precipitate, or adding other reagents to form complex ions.
For instance, group IV...
24.5K
Dimensional Analysis03:40

Dimensional Analysis

64.8K
Dimensional analysis, also known as the factor label method, is a versatile approach for mathematical operations. The main principle behind this approach is: the units of quantities must be subjected to the same mathematical operations as their associated numbers. This method can be applied to computations ranging from simple unit conversions to more complex and multi-step calculations involving several different quantities and their units.
Conversion Factors and Dimensional Analysis
The unit...
64.8K
Dimensional Analysis01:27

Dimensional Analysis

681
Dimensional analysis is a valuable technique in fluid mechanics for simplifying complex problems by reducing them into dimensionless groups. These groups capture the essential relationships between the variables involved, allowing researchers and engineers to analyze fluid flow without dealing with each variable individually. This approach reduces the number of independent variables, allowing for easier analysis and better understanding of physical phenomena.
In fluid mechanics, dimensional...
681
Pedigree Analysis01:35

Pedigree Analysis

89.5K
Overview
89.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Preventable Congenital Heart Malformation Syndrome Caused by a Mutation in the Glycolytic Gene PFKP.

JACC. Basic to translational science·2026
Same author

Epigenetic safety of in vitro maturation in PCOS: genome-wide DNA methylation profiling of cord blood from a randomized controlled trial.

Journal of ovarian research·2026
Same author

Authenticity in Virtual and Human Influencer Donation Appeals: Effects of Emotional Expression and Language Style.

Cyberpsychology, behavior and social networking·2026
Same author

A tumor-resident <i>Staphylococcus epidermidis</i> strain exhibits genomic and metabolic traits driving hepatocellular carcinoma progression.

iScience·2026
Same author

Third-generation sequencing enables accurate preimplantation genetic testing of monogenic defects for de novo variants and incomplete pedigrees.

Human reproduction (Oxford, England)·2026
Same author

How I Treat: Haploinsufficiency of A20.

Journal of human immunity·2026

Related Experiment Video

Updated: Feb 5, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
07:24

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

2.0K

Pyrosequencing Methylation Analysis.

Matthew Poulin1, Jeffrey Y Zhou2, Liying Yan3

  • 1EpigenDx, Inc., Hopkinton, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 5, 2018
PubMed
Summary

Quantitative bisulfite Pyrosequencing offers a gold standard for DNA methylation analysis, providing accurate and reproducible measurements for clinical research and drug treatment studies.

Keywords:
Bisulfite PCRDNA methylationHypomethylationPyrosequencingResponse to drug treatmentTumor suppressor genesWhole-genome methylation

More Related Videos

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
13:21

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

10.5K
DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

106.5K

Related Experiment Videos

Last Updated: Feb 5, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
07:24

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

2.0K
Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
13:21

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

10.5K
DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

106.5K

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Genomics

Background:

  • Quantitative bisulfite Pyrosequencing is a gold standard for allele quantification.
  • It determines DNA methylation levels by analyzing C/T SNPs at CpG sites.

Purpose of the Study:

  • To highlight the accuracy and reproducibility of Pyrosequencing for DNA methylation analysis.
  • To demonstrate its suitability for clinical research, validation, and global methylation studies.

Main Methods:

  • Designing strand-specific Pyrosequencing methylation assays without CpG sites in primers.
  • Testing assays for preferential amplification during bisulfite PCR.
  • Analyzing methylation at multiple CpG sites from PCR products.

Main Results:

  • Pyrosequencing provides rapid, accurate, and reproducible measurements of average methylation.
  • Low measurement variance increases the likelihood of detecting small methylation changes.
  • Assays are suitable for repetitive elements like LINE-1, Alu, and Sat2.

Conclusions:

  • Pyrosequencing is well-suited for clinical research, validating screening results, and global methylation analysis.
  • Its accuracy and reproducibility aid in detecting methylation changes in response to treatment and disease.
  • Automation and cost-effectiveness enable larger sample populations for statistical significance.