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Polymorphonuclear leukocyte response to stimulation in vitro during pregnancy
Inflammation
|September 1, 1986
Summary
Pregnancy alters polymorphonuclear leukocyte (PMNL) oxidative metabolism, increasing chemiluminescence but decreasing superoxide generation. PMNL degranulation capacity remains unchanged, suggesting in vivo stimulation during pregnancy.
Area of Science:
- Immunology
- Perinatal Medicine
- Cellular Metabolism
Background:
- Polymorphonuclear leukocytes (PMNLs) play a crucial role in innate immunity.
- Changes in PMNL function during pregnancy are not fully understood.
- Investigating PMNL oxidative metabolism is key to understanding immune adaptations during gestation.
Purpose of the Study:
- To investigate the oxidative metabolism of PMNLs in pregnant women during the third trimester.
- To compare PMNL function between pregnant women and non-pregnant controls.
- To elucidate potential alterations in PMNL activity during pregnancy.
Main Methods:
- Isolation of PMNLs from pregnant women (third trimester) and control subjects.
- Measurement of zymosan-induced chemiluminescence (CL).
- Assessment of f-Met-Leu-Phe-stimulated superoxide (O2-) generation and cytochrome c reduction.
Main Results:
- Significantly increased CL was observed in PMNLs from pregnant women.
- A decrease in cytochrome c reduction was noted during pregnancy.
- Levels of beta-glucuronidase and lysozyme were diminished, while lactate dehydrogenase remained unaltered.
Conclusions:
- PMNL oxidative metabolism is altered during the third trimester of pregnancy.
- Despite functional changes, PMNL degranulation capacity is preserved.
- Observed alterations may indicate in vivo stimulation of PMNLs during pregnancy.