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Quality control is one of the three cyclical quality assurance activities that help keep a system under statistical control. Typical quality control activities include creating quality control charts, conducting proficiency testing, and documenting and archiving results.
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A new high-throughput method to make a quality control on tattoo inks.

S Persechino1, C Toniolo2, A Ciccola3

  • 1NESMOS Department - Dermatology Unit of S. Andrea Hospital, Sapienza University of Rome, Italy.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|September 5, 2018
PubMed
Summary

A new method using High-Performance Thin-Layer Chromatography-Raman (HPTLC-Raman) spectroscopy enhances tattoo ink quality control. This technique identifies tattoo pigment components, aiding in the prevention of adverse reactions and improving patient safety.

Keywords:
HPTLC-RamanPigmentsTattoo inks

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Area of Science:

  • Analytical Chemistry
  • Dermatology
  • Materials Science

Background:

  • Tattooing is increasingly popular globally, leading to a rise in adverse reactions from tattoo pigments.
  • Cutaneous and allergic reactions, including contact dermatitis, are documented side effects linked to specific tattoo pigments.
  • Existing quality control methods for tattoo inks may not adequately identify all potentially harmful components.

Purpose of the Study:

  • To introduce a novel, high-throughput method for the quality control of tattoo inks.
  • To develop a system for identifying the specific chemical constituents within tattoo inks.
  • To establish a tool for detecting substances in tattoo inks that may cause adverse reactions.

Main Methods:

  • A chromatographic-spectroscopic approach combining High-Performance Thin-Layer Chromatography (HPTLC) with Raman spectroscopy was employed.
  • Twenty-four samples, including 21 tattoo inks and 3 permanent makeup products in various colors, were analyzed.
  • The HPTLC-Raman technique was utilized for its sensitivity to both inorganic and organic pigments, separating complex mixtures into individual components for spectral analysis.

Main Results:

  • The HPTLC-Raman method successfully separated and analyzed individual components within complex tattoo ink mixtures.
  • Raman spectroscopy provided detailed spectral information for each isolated pigment, enabling identification of its chemical nature.
  • The technique demonstrated high sensitivity towards the inorganic and organic pigments commonly found in tattoo inks.

Conclusions:

  • The developed HPTLC-Raman method offers a powerful tool for the quality control of tattoo inks.
  • This approach can accurately identify tattoo ink components, including those potentially responsible for allergic reactions.
  • The system has the potential to significantly improve safety for individuals receiving tattoos by ensuring ink quality and identifying harmful substances.