Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

16.8K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.8K
Protein Complex Assembly02:41

Protein Complex Assembly

2.6K
2.6K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

21.0K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
21.0K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

6.6K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
6.6K
Assembly of Complex Microtubule Structures01:32

Assembly of Complex Microtubule Structures

2.5K
Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
2.5K
Genomics02:02

Genomics

40.7K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Generation of a Commercial-Scale Founder Population of Porcine Reproductive and Respiratory Syndrome Virus Resistant Pigs Using CRISPR-Cas.

The CRISPR journal·2024
Same author

Intragenic Meiotic Crossovers Generate Novel Alleles with Transgressive Expression Levels.

Molecular biology and evolution·2018
Same author

tGBS® genotyping-by-sequencing enables reliable genotyping of heterozygous loci.

Nucleic acids research·2017
Same author

Molecular Mapping of D₁, D₂ and ms5 Revealed Linkage between the Cotyledon Color Locus D₂ and the Male-Sterile Locus ms5 in Soybean.

Plants (Basel, Switzerland)·2016
Same author

Molecular mapping of five soybean genes involved in male-sterility, female-sterility.

Genome·2015
Same author

Evolutionary, Comparative and Functional Analyses of the Brassinosteroid Receptor Gene, BRI1, in Wheat and Its Relation to Other Plant Genomes.

PloS one·2015
Same journal

Chromosomal scale genome assembly of medicinal plant Sophora tonkinensis.

BMC genomics·2026
Same journal

Variant-specific RNA testing resolves variants of uncertain significance in exome testing.

BMC genomics·2026
Same journal

Kaiso overexpression promotes an interferon immune response in murine intestines.

BMC genomics·2026
Same journal

Genomic evidence of ecological flexibility and cross-niche CRISPR spacerome targeting phage-plasmid hybrids in Latilactobacillus curvatus.

BMC genomics·2026
Same journal

Fgf evolution in vertebrates: insights from cyclostomes.

BMC genomics·2026
Same journal

Metabolic reprogramming, oxidative stress, and mitophagy in JSRV Env-transformed BEAS-2B cells: insights from integrated transcriptomics and metabolomics.

BMC genomics·2026
See all related articles

Related Experiment Video

Updated: Feb 5, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.8K

Linked read technology for assembling large complex and polyploid genomes.

Alina Ott1,2, James C Schnable3,4,5, Cheng-Ting Yeh1,4

  • 1Department of Agronomy, Iowa State University, Ames, IA, 50011, USA.

BMC Genomics
|September 6, 2018
PubMed
Summary
This summary is machine-generated.

Linked read technology improves genome assembly for complex plant genomes. This method successfully assembled maize and proso millet, offering a framework for future repetitive genome projects.

Keywords:
Genome assemblyLong molecule sequencingPolyploid assembly

More Related Videos

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

24.0K
Amplicon Sequencing using the Long-Read Sequencing Technologies
08:57

Amplicon Sequencing using the Long-Read Sequencing Technologies

Published on: August 29, 2025

537

Related Experiment Videos

Last Updated: Feb 5, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.8K
Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

24.0K
Amplicon Sequencing using the Long-Read Sequencing Technologies
08:57

Amplicon Sequencing using the Long-Read Sequencing Technologies

Published on: August 29, 2025

537

Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Short-read DNA sequencing is cost-effective but struggles with complex genomes.
  • Linked reads offer enhanced genome assembly by barcoding DNA fragments from single long molecules.
  • Previous linked-read applications primarily focused on human genome phasing.

Purpose of the Study:

  • To evaluate linked read technology for de novo genome assembly of complex plant genomes.
  • To assess the effectiveness of linked reads in resolving polyploid genomes.
  • To develop computational strategies for improving linked read assemblies.

Main Methods:

  • Utilized linked read technology for de novo genome assembly.
  • Applied machine learning for error identification in assembled contigs.
  • Performed comparative analysis against existing reference genomes.

Main Results:

  • Generated a de novo maize B73 genome assembly with 172,000 scaffolds (N50 89 kb).
  • Achieved 91% accuracy in linked read contigs compared to the B73 reference.
  • Successfully resolved the two subgenomes of the allopolyploid proso millet (Panicum miliaceum).

Conclusions:

  • Linked read technology provides a viable approach for assembling complex plant genomes.
  • Machine learning can identify errors in linked read assemblies with high accuracy.
  • Computational strategies are proposed to further enhance linked read assembly, especially for repetitive genomes.