Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

15.2K
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
15.2K
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

4.5K
4.5K
Regulated Protein Degradation02:58

Regulated Protein Degradation

8.9K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
8.9K
Regulated Protein Degradation02:58

Regulated Protein Degradation

3.2K
3.2K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

9.6K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
9.6K
Positive Regulator Molecules01:45

Positive Regulator Molecules

136.4K
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
136.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A control theoretical approach to gene regulation reveals quantitative constraints for dynamic homeostasis in stochastic gene expression.

Biochimica et biophysica acta. Molecular basis of disease·2026
Same author

Protein Kinase A Phosphorylates a Conformationally High-energy State of Raf Kinase Inhibitory Protein.

Journal of molecular biology·2025
Same author

Direct inhibition of tumor hypoxia response with synthetic transcriptional repressors.

Nature chemical biology·2024
Same author

Nonmonotone invasion landscape by noise-aware control of metastasis activator levels.

Nature chemical biology·2023
Same author

SARS-CoV-2 Diverges from Other Betacoronaviruses in Only Partially Activating the IRE1α/XBP1 Endoplasmic Reticulum Stress Pathway in Human Lung-Derived Cells.

mBio·2022
Same author

SARS-CoV-2 diverges from other betacoronaviruses in only partially activating the IRE1α/XBP1 ER stress pathway in human lung-derived cells.

bioRxiv : the preprint server for biology·2022

Related Experiment Video

Updated: Feb 5, 2026

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods
07:49

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods

Published on: July 17, 2019

6.5K

Targeting Raf Kinase Inhibitory Protein Regulation and Function.

Ali Ekrem Yesilkanal1,2, Marsha Rich Rosner3,4

  • 1Ben May Department for Cancer Research, University of Chicago, Chicago, IL 60637, USA. aeyesilkanal@gmail.com.

Cancers
|September 6, 2018
PubMed
Summary

Raf Kinase Inhibitory Protein (RKIP) suppresses cancer metastasis by reprogramming tumor cells. Understanding RKIP loss in tumors offers therapeutic strategies targeting metastasis mediators.

Keywords:
RKIPkinasemetastasissignalingsuppressortherapy

More Related Videos

Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes
15:43

Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes

Published on: January 7, 2013

18.6K
A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
07:18

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Published on: May 12, 2017

6.8K

Related Experiment Videos

Last Updated: Feb 5, 2026

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods
07:49

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods

Published on: July 17, 2019

6.5K
Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes
15:43

Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes

Published on: January 7, 2013

18.6K
A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
07:18

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Published on: May 12, 2017

6.8K

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Raf Kinase Inhibitory Protein (RKIP) is a conserved protein that acts as a metastasis suppressor in various cancers.
  • RKIP can alter tumor cells to a non-metastatic state by modifying kinase networks, offering therapeutic targets.
  • Loss of RKIP expression is frequently observed during cancer progression, but the underlying mechanisms remain unclear.

Purpose of the Study:

  • To review the current understanding of RKIP regulation in tumors.
  • To explore experimental and computational approaches for restoring or mimicking RKIP function.
  • To identify therapeutic strategies targeting metastasis mediators.

Main Methods:

  • Literature review of RKIP function and regulation in cancer.
  • Analysis of mechanisms underlying RKIP loss during metastasis.
  • Discussion of therapeutic strategies targeting RKIP-mediated signaling pathways.

Main Results:

  • RKIP's role as a metastasis suppressor through kinase network rewiring is highlighted.
  • The complex and not fully understood mechanisms of RKIP loss in tumor cells are discussed.
  • Potential strategies for recovering or mimicking RKIP function are considered.

Conclusions:

  • Elucidating RKIP's mechanism provides insights into metastasis regulation.
  • Targeting RKIP signaling networks offers a therapeutic opportunity for cancer treatment.
  • Further research into RKIP regulation and therapeutic strategies is warranted.