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Updated: Feb 5, 2026

Analysis of Lymph Node Volume by Ultra-High-Frequency Ultrasound Imaging in the Braf/Pten Genetically Engineered Mouse Model of Melanoma
Published on: September 8, 2021
Accelerated BRAF mutation analysis using a fully automated PCR platform improves the management of patients with
Delphine Serre1, Julia Salleron2, Marie Husson3
1Institut de Cancérologie de Lorraine, Département d'Oncologie Médicale, Vandoeuvre-lès-Nancy Cedex 54519, France.
Background:
Determination of BRAF status is important for the therapeutic management of patients with metastatic melanoma.
Objectives:
We evaluated the impact of a faster determination of BRAF mutational status on the delay between initial consultation and initiation of treatment.
Results:
For the FA-PCR group a median delay of 16 days [11;18] was observed between initial consultation and the implementation of treatment, which was significantly lower than that observed for the SOP group (26 days [20;46], p = 0.035).
Conclusions:
In comparison to using conventional SOP, using an FA-PCR platform for BRAF mutation analysis of patients with metastatic melanoma significantly reduced the delay in initiation of personalized therapy by 10 days.
Materials And Methods:
Analysis of the BRAF mutation status of eight formalin-fixed paraffin-embedded (FFPE) tissue samples was performed using a CE-IVD fully-automated (FA) PCR-based platform. The delay between initial consultation and the implementation of treatment was compared between these samples (FA-PCR group) and a retrospective group of 29 FFPE samples analysed by standard operating procedures (SOP group) using conventional PCR.
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