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The Use of Drip Flow and Rotating Disk Reactors for Staphylococcus aureus Biofilm Analysis
Published on: December 27, 2010
MLST and RAPD molecular analysis of Staphylococcus aureus subsp. anaerobius isolated from goats in Poland
Olga Szaluś-Jordanow1, Katarzyna Krysztopa-Grzybowska2, Michał Czopowicz3
1Division of Infectious Diseases, Department of Small Animal Diseases with Clinic, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159c Street, 02-776, Warsaw, Poland. olga_szalus@sggw.pl.
Abstract:
Staphylococcus aureus subsp. anaerobius is an etiological agent of Morel's disease in small ruminants. The infection results in superficial abscesses located near lymph nodes. In the study, molecular analysis based on multilocus sequence typing (MLST) of seven housekeeping genes (arcC, aroE, glp, gmk, pta, tpi, yqiL) and random amplified polymorphic DNA (RAPD) was carried out on 19 S. aureus subsp. anaerobius strains isolated from two different goat herds from Poland. All of the 19 S. aureus subsp. anaerobius strains were found to belong to single MLST and RAPD types which support the high clonality level of this agent. However, the results obtained show clearly that the S. aureus subsp. anaerobius clone found in goats in Poland is different from those previously described. However, it is identical to the ATCC 38844 strain isolated from sheep in Spain, which has not been so far genotyped using MLST.
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