Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Profile Leveling and Cross Sections01:26

Profile Leveling and Cross Sections

1.6K
Profile leveling and cross-sections are surveying methods used to determine and document terrain elevations for infrastructure projects such as highways, railroads, canals, and pipelines. These methods provide data for earthwork planning and alignment of proposed routes.  Profile leveling involves measuring elevations along a fixed line to create a vertical terrain profile. A surveyor sets up a leveling instrument at the benchmark (BM) and records a backsight (BS) to determine the...
1.6K
Taping Over Different Ground Profiles01:12

Taping Over Different Ground Profiles

358
Taping over varying ground profiles requires careful adaptation to achieve accurate measurements. On smooth, level ground with minimal vegetation, the tape can rest directly on the ground. Here, the taping team, typically consisting of a head and a rear tapeman, coordinates their positions with clear communication. The rear tapeman holds the tape at the starting point and guides the head tapeman toward a range pole placed beyond the endpoint, using hand or voice signals to ensure alignment.On...
358
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
Geometric Mean01:15

Geometric Mean

3.9K
The mean is a measure of the central tendency of a data set. In some data sets, the data is inherently multiplicative, and the arithmetic mean is not useful. For example, the human population multiplies with time, and so does the credit amount of financial investment, as the interest compounds over successive time intervals.
In cases of multiplicative data, the geometric mean is used for statistical analysis. First, the product of all the elements is taken. Then, if there are n elements in the...
3.9K
Ribosome Profiling02:24

Ribosome Profiling

4.1K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.1K
Chromatographic Resolution01:15

Chromatographic Resolution

2.1K
In chromatography, a solute moves through a chromatographic column and tends to spread, forming a Gaussian-shaped band. The longer the solute spends in the column, the broader the band becomes. The broadening can lead to overlaps within the column, affecting separation effectiveness.
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Surface cross-linked thermoplastic starch with different UV wavelengths: mechanical, wettability, hygroscopic and degradation properties.

RSC advances·2022
Same author

A novel method to produce sustainable biocomposites based on thermoplastic corn-starch reinforced by polyvinyl alcohol fibers.

RSC advances·2022
Same author

Mortality and Years of Life Lost in Diabetes Mellitus and Its Subcategories in China and Its Provinces, 2005-2020.

Journal of diabetes research·2022
Same author

Sub-3-Å cryo-EM structure of RNA enabled by engineered homomeric self-assembly.

Nature methods·2022
Same author

Spatial variations and social determinants of life expectancy in China, 2005-2020: A population-based spatial panel modelling study.

The Lancet regional health. Western Pacific·2022
Same author

Pentacoordinate Al<sup>3+</sup> Sites Anchoring Synthesis of Palladium Intermetallic Catalysts on Al<sub>2</sub>O<sub>3</sub> Supports.

Inorganic chemistry·2022
Same journal

Efficient Syngas Photoproduction Enabled by Electronic Engineering of Co-Immobilized Imine COFs.

Angewandte Chemie (International ed. in English)·2026
Same journal

Pathway Controlled Phase Separation of Minimal Building Blocks Utilizing a Dissociative Chemical Transformation.

Angewandte Chemie (International ed. in English)·2026
Same journal

Interaction Hierarchy and Polymorphic Structure-Property Dynamics in Luminescent Molecular Crystals.

Angewandte Chemie (International ed. in English)·2026
Same journal

The Role of Zn-Hf Site Proximity and Oxygen Vacancies for Methanol Formation Over ZnHfO<sub>x</sub> Catalysts Under CO<sub>2</sub> Hydrogenation Conditions.

Angewandte Chemie (International ed. in English)·2026
Same journal

Breaking the Linear Scaling Relationship: Bioinspired Electronic Coupling in S-Bridged Fe-Fe Dual Sites for Efficient Oxygen Reduction.

Angewandte Chemie (International ed. in English)·2026
Same journal

Programming Bio-Bio Electronic Interfaces for Light-Driven Interspecies Electron Transfer.

Angewandte Chemie (International ed. in English)·2026
See all related articles

Related Experiment Video

Updated: Jan 26, 2026

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
08:43

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

7.8K

Super-resolution Geometric Barcoding for Multiplexed miRNA Profiling.

Weidong Xu1,2,3, Peng Yin1,2, Mingjie Dai1,2

  • 1Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA.

Angewandte Chemie (International Ed. in English)
|September 6, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces a super-resolution imaging technique for precise microRNA (miRNA) detection. The method enables sensitive, specific, and multiplexed profiling of miRNA biomarkers for disease diagnostics and prognosis.

Keywords:
DNA nanotechnologyDNA-PAINTmicroRNAmultiplexingsuper-resolution imaging

More Related Videos

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

9.7K
Super-resolution Imaging of Neuronal Dense-core Vesicles
09:30

Super-resolution Imaging of Neuronal Dense-core Vesicles

Published on: July 2, 2014

10.1K

Related Experiment Videos

Last Updated: Jan 26, 2026

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
08:43

Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Published on: June 24, 2017

7.8K
Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

9.7K
Super-resolution Imaging of Neuronal Dense-core Vesicles
09:30

Super-resolution Imaging of Neuronal Dense-core Vesicles

Published on: July 2, 2014

10.1K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Nanotechnology

Background:

  • MicroRNA (miRNA) expression profiles are crucial for disease diagnostics and prognosis.
  • Existing methods for miRNA detection face challenges in specificity, sensitivity, and multiplexing capabilities.

Purpose of the Study:

  • To develop a super-resolution fluorescence imaging-based digital profiling method for specific, sensitive, and multiplexed miRNA detection.
  • To demonstrate the capability of this method for analyzing both synthetic and endogenous miRNA samples.

Main Methods:

  • Utilized DNA-PAINT (point accumulation for imaging in nanoscale topography) for super-resolution imaging.
  • Implemented a geometric barcoding scheme with synthetic DNA nanostructures for multiplexed single-molecule miRNA capture and digital counting.
  • Applied an 8-plex panel to measure endogenous miRNA levels in HeLa cell total RNA extracts.

Main Results:

  • Achieved high specificity with single nucleotide mismatch discrimination.
  • Demonstrated multiplexed detection capabilities (8-plex, 2 panels) for synthetic miRNA samples.
  • Successfully measured endogenous miRNA levels in a biological sample.

Conclusions:

  • The developed super-resolution imaging method offers a powerful tool for precise miRNA profiling.
  • This technique shows significant potential for advancing miRNA-based diagnostics and prognostics in complex diseases.