Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The DNA Helix01:16

The DNA Helix

157.6K
Overview
157.6K
The DNA Helix01:07

The DNA Helix

30.5K
Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
30.5K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

9.5K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.5K
Distance Corrections01:15

Distance Corrections

293
To achieve precise distance measurements, especially in surveying and construction, certain corrections must be applied to account for potential sources of error like the standardization errors, temperature variations, and slope adjustments.Standardization error emerges when measurement equipment undergoes changes, such as wear, repairs, or weather impacts. To address this, surveyors compare the equipment’s readings to a standard. This process identifies any deviation that might lead to...
293
Power Factor Correction01:20

Power Factor Correction

544
The power transmission to a factory involves the transfer of apparent power, a combination of active and reactive power. The power factor measures how effectively electrical power is converted into useful work output. The ratio of the real power (KW) that does the work to the apparent power (KVA) supplied to the circuit.
544
DNA Base Pairing02:27

DNA Base Pairing

33.3K
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
33.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-fidelity compressed high-speed imaging for resolving rapid micro-dynamics.

Optics express·2026
Same author

Lensless quantitative microscopy based on multi-angle illumination and coded ptychographic phase retrieval.

Optics letters·2026
Same author

Scalable generalized meta-spanners enabling parallel multitasking optical manipulation.

Science advances·2026
Same author

Large-Aperture Polarization-Independent Broadband Achromatic All-Dielectric Metalens for Terahertz Focusing.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Comprehensive Identification of the Bovine KLF Gene Family and Its Functional Regulation in Muscle Development: Insights from Single-Nuclei Transcriptomics.

Animals : an open access journal from MDPI·2025
Same author

High-Precision Refractive Index-Based Microparticle Sorting Using Airy Beams.

Analytical chemistry·2025
Same journal

Method of spatial scanning of modulated laser radiation for outline imaging of interphalangeal joints.

Journal of biomedical optics·2026
Same journal

Multimodal optical imaging for the assessment of the teratogenic effects of ethanol on zebrafish development.

Journal of biomedical optics·2026
Same journal

Fluorescence properties of collagen types I-V: a comprehensive study of spectral and lifetime characteristics.

Journal of biomedical optics·2026
Same journal

Spectral dependence of lipofuscin fluorescence lifetimes revealed by FLIM with a superconducting nanowire single-photon detector.

Journal of biomedical optics·2026
Same journal

Building the future of biophotonics through experiential education and seasonal schools.

Journal of biomedical optics·2026
Same journal

Time-of-flight fluorescence depth mapping using a spatiotemporal deep learning model.

Journal of biomedical optics·2026
See all related articles

Related Experiment Video

Updated: Feb 5, 2026

Single-Digit Nanometer Electron-Beam Lithography with an Aberration-Corrected Scanning Transmission Electron Microscope
10:25

Single-Digit Nanometer Electron-Beam Lithography with an Aberration-Corrected Scanning Transmission Electron Microscope

Published on: September 14, 2018

10.7K

Aberration correction method based on double-helix point spread function.

Zhaojun Wang1,2, Yanan Cai1,2, Yansheng Liang1,2

  • 1Chinese Academy of Sciences, Xi'an Institute of Optics and Precision Mechanics, State Key Laboratory, China.

Journal of Biomedical Optics
|September 6, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces an iteration-free method to correct optical aberrations in point spread function (PSF) engineering. The double-helix PSF serves as an aberration indicator, improving image quality in microscopy and tracking applications.

Keywords:
aberration correctiondouble-helix point spread functioniteration-free

More Related Videos

Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System
08:48

Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System

Published on: December 21, 2016

9.2K
Author Spotlight: Advancements in Refractive Surgical Correction for Presbyopia and Exploring Postoperative Visual Acuity
05:46

Author Spotlight: Advancements in Refractive Surgical Correction for Presbyopia and Exploring Postoperative Visual Acuity

Published on: September 20, 2024

825

Related Experiment Videos

Last Updated: Feb 5, 2026

Single-Digit Nanometer Electron-Beam Lithography with an Aberration-Corrected Scanning Transmission Electron Microscope
10:25

Single-Digit Nanometer Electron-Beam Lithography with an Aberration-Corrected Scanning Transmission Electron Microscope

Published on: September 14, 2018

10.7K
Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System
08:48

Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System

Published on: December 21, 2016

9.2K
Author Spotlight: Advancements in Refractive Surgical Correction for Presbyopia and Exploring Postoperative Visual Acuity
05:46

Author Spotlight: Advancements in Refractive Surgical Correction for Presbyopia and Exploring Postoperative Visual Acuity

Published on: September 20, 2024

825

Area of Science:

  • Optical imaging
  • Microscopy
  • Biophysics

Background:

  • Point spread function (PSF) engineering is crucial for advanced optical imaging techniques.
  • System aberrations degrade image quality, reducing contrast, resolution, and localization precision.
  • Current aberration correction methods are often iterative and computationally intensive.

Purpose of the Study:

  • To develop an efficient, iteration-free method for correcting system aberrations in PSF engineering.
  • To utilize a spiral-phase-based double-helix PSF as a sensitive indicator for aberrations.
  • To enable direct calculation of aberration coefficients without complex iterative processes.

Main Methods:

  • Employing a double-helix PSF engineered with spiral phase elements to probe system aberrations.
  • Quantitatively correlating the PSF's intensity distribution with specific aberrations (spherical aberration, coma, astigmatism).
  • Directly calculating aberration coefficients from measured parameters, bypassing iterative algorithms.

Main Results:

  • Demonstrated the sensitivity of the double-helix PSF to spherical aberration, coma, and astigmatism.
  • Validated the iteration-free correction method through simulations and experimental imaging of cells.
  • Showcased the method's ability to accurately determine aberration coefficients.

Conclusions:

  • The proposed iteration-free aberration correction method effectively utilizes a double-helix PSF as an aberration indicator.
  • This approach significantly reduces computational burden compared to traditional methods.
  • The technique holds potential for real-time aberration correction in PSF engineering systems with spatial light modulators.