Circular RNA hsa_circ_0072995 promotes breast cancer cell migration and invasion through sponge for miR-30c-2-3p

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He-Da Zhang1,2, Lin-Hong Jiang3, Jun-Chen Hou4

  • 1Department of General Surgery, School of Medicine, Southeast University, Nanjing, Jiangsu, PR China.

Epigenomics
|September 6, 2018
PubMed

Insights

This study reveals that hsa_circ_0072995 is upregulated in breast cancer cells and promotes their invasion and migration. It interacts with miR-30c-2-3p, suggesting a role in breast cancer metastasis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Breast cancer is a leading cause of cancer-related mortality worldwide.
  • Circular RNAs (circRNAs) are emerging as critical regulators in various cancers, including breast cancer.
  • Understanding the specific roles of circRNAs in breast cancer progression is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate the function of hsa_circ_0072995 in regulating breast cancer cell invasion and migration.
  • To explore the molecular mechanisms underlying the role of hsa_circ_0072995 in breast cancer.
  • To assess the potential of hsa_circ_0072995 as a biomarker for breast cancer.

Main Methods:

  • Quantitative real-time PCR (qPCR) was used to confirm hsa_circ_0072995 expression levels.
  • Transwell assays were employed to evaluate breast cancer cell migration and invasion.
  • Bioinformatic tools (miRanda, RNAhybrid) predicted circRNA/microRNA interactions.
  • Luciferase activity assays identified the interaction between hsa_circ_0072995 and miR-30c-2-3p.
  • Fluorescence in situ hybridization (FISH) determined the subcellular localization of hsa_circ_0072995.

Main Results:

  • Hsa_circ_0072995 was significantly upregulated in MDA-MB-231 breast cancer cells compared to MCF-7 cells.
  • Hsa_circ_0072995 was found to regulate the invasion and migration of breast cancer cells.
  • Hsa_circ_0072995 was detected in both the nucleus and cytoplasm of breast cancer cells.
  • Hsa_circ_0072995 directly binds to miR-30c-2-3p.
  • Overexpression of miR-30c-2-3p inhibited breast cancer cell migration and invasion.
  • Low miR-30c-2-3p expression correlated with poorer overall survival in breast cancer patients, based on The Cancer Genome Atlas (TCGA) database.

Conclusions:

  • Hsa_circ_0072995 plays a significant role in promoting breast cancer cell invasion and migration.
  • The interaction between hsa_circ_0072995 and miR-30c-2-3p is a key mechanism in breast cancer progression.
  • Hsa_circ_0072995 may serve as a novel diagnostic biomarker and therapeutic target for breast cancer metastasis.
Abstract

Keywords:
breast cancercircRNAshsa_circ_0072995miRNA-30c-2-3p

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