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07:29
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
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Effect of some phytoconstituents on Fe2+/ascorbate induced lipid peroxidation
Indian Journal of Experimental Biology
|September 6, 2018
Summary
Certain plant compounds can protect against oxidative stress by reducing lipid peroxidation. Some phytoconstituents, like acteoside and berberine, showed antioxidant properties, while others increased oxidative damage, highlighting their varied effects on reactive oxygen species (ROS).
Area of Science:
- Biochemistry
- Pharmacology
- Oxidative Stress Research
Background:
- Transition metals (iron, copper) catalyze reactive oxygen species (ROS) production.
- ROS cause lipid peroxidation, damaging cellular structures and leading to disease.
- Plant-derived antioxidants are investigated for cellular protection against ROS.
Purpose of the Study:
- To evaluate the ROS scavenging and lipid peroxidation inhibitory effects of specific phytoconstituents.
- To assess the impact of selected medicinal plant compounds on oxidative damage in rat liver homogenate.
Main Methods:
- Lipid peroxidation was measured using the thiobarbituric acid reaction in rat liver homogenate.
- Basal and Fe2+/ascorbate-induced lipid peroxidation were assessed.
- The effects of various phytoconstituents on these peroxidation levels were determined.
Main Results:
- Fe2+/ascorbate system significantly induced lipid peroxidation.
- Acteoside, berberine, catechin, a flavonoid glycoside from cumin, silibin, and tetrahydrocurcumin inhibited both basal and induced lipid peroxidation.
- Agnuside, andrographolide, picroside-I, negunoside, oleanolic acid, and glycerrihizin enhanced lipid peroxidation.
Conclusions:
- Certain phytoconstituents exhibit significant antioxidant activity by reducing lipid peroxidation.
- These protective compounds may offer defense against ROS-mediated damage and associated diseases.
- Other tested phytoconstituents may exacerbate oxidative stress, necessitating careful evaluation.
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