Training tuberculosis laboratory workers in LED-fluorescence microscopy: experience learned in Argentina

Area of Science:

• Microbiology
• Medical Laboratory Science

Background:

• LED-fluorescence microscopy (LED-FM) is a crucial diagnostic tool.
• Training laboratory technicians without prior FM experience requires effective capacitation programs.

Purpose of the Study:

• To evaluate a LED-FM training program for novice laboratory technicians.
• To determine the necessary duration and conditions for achieving proficiency in LED-FM.

Main Methods:

• A three-day course followed by a two-month "in situ" training phase without expert supervision.
• Technicians examined slides stained with Ziehl Neelsen (ZN) and FM, progressing to blinded testing.
• Proficiency was assessed using testing panels and a rechecking process.

Main Results:

• Initially, 70% of trainees made Low False Positive errors (LFPs), which decreased significantly with "in situ" training.
• Proficiency was achieved with at least three slides examined daily.
• During blinded training, sensitivity reached 96.8% and specificity 99.8%.

Conclusions:

• A brief LED-FM course alone does not ensure technician proficiency.
• Effective skill acquisition in LED-FM is possible through a short "in situ" phase, even without on-site expert support.
• Training effectiveness is linked to a minimum workload, with 750 slides/year recommended.