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Related Concept Videos

Nucleic Acids02:43

Nucleic Acids

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
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Nucleic acids02:43

Nucleic acids

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
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Nucleic Acids02:43

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Nucleic Acid Structure01:25

Nucleic Acid Structure

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The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
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Nucleic Acids and Nucleotides01:20

Nucleic Acids and Nucleotides

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and have instructions for its functioning. The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).
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Biosynthesis of Nucleic Acids01:28

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Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
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Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
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Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

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Polyester-based nanoparticles for nucleic acid delivery.

Jing Zhao1, Guojun Weng1, Jianjun Li1

  • 1The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China.

Materials Science & Engineering. C, Materials for Biological Applications
|September 7, 2018
PubMed
Summary
This summary is machine-generated.

Gene therapy utilizes nanoparticle delivery systems to protect and deliver various nucleic acids for treating diseases. Cationic polyesters show promise for efficient gene delivery, enhancing therapeutic outcomes.

Keywords:
Gene deliveryGene therapyNanoparticlePolyesterRNA delivery

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Modulating Shape of Polyester Based Polymersomes using Osmotic Pressure
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Area of Science:

  • Biotechnology and Biomedical Engineering
  • Materials Science in Medicine
  • Molecular and Cellular Biology

Background:

  • Gene therapy offers potential for treating inherited and complex diseases like cancer and infections.
  • Therapeutic scope expanded from gene transfer to delivering diverse nucleic acids, including mRNA, ASOs, siRNA, and miRNA.
  • Nanoparticle delivery systems are crucial for protecting nucleic acids from degradation and immune responses.

Purpose of the Study:

  • To explore the role of nanoparticle delivery systems in advancing gene therapy.
  • To investigate the application of aliphatic polyesters in nucleic acid delivery.
  • To highlight the synthesis of cationic polyester nanostructures for efficient gene delivery.

Main Methods:

  • Utilized nanoparticle delivery systems for enhanced protection and intracellular transport of nucleic acids.
  • Employed aliphatic polyesters (PLA, PLGA, PCL, PHB) known for biocompatibility and degradability.
  • Synthesized cationic polyester nanospheres, micelles, and dendrimers through physical mixing, chemical conjugation, and copolymerization.

Main Results:

  • Nanoparticle systems demonstrated efficient protection of nucleic acids against enzymatic degradation and immune recognition.
  • These systems facilitated intracellular delivery and evasion of renal/hepatic clearance for sustained tissue targeting.
  • Cationic polyester nanostructures effectively condensed and delivered various nucleic acids.

Conclusions:

  • Nanoparticle delivery systems are vital for the efficacy and stability of gene therapy agents.
  • Aliphatic polyesters offer a biocompatible and degradable platform for developing advanced gene delivery vehicles.
  • Cationic polyester-based nanostructures represent a promising approach for targeted and efficient gene therapy applications.