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Related Experiment Video

Updated: Feb 5, 2026

Novel Photoacoustic Microscopy and Optical Coherence Tomography Dual-modality Chorioretinal Imaging in Living Rabbit Eyes
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Coherence-gated photoacoustic remote sensing microscopy.

Kevan L Bell, Parsin Hajireza, Roger J Zemp

    Optics Express
    |September 7, 2018
    PubMed
    Summary

    Photoacoustic remote sensing microscopy (PARS) now offers depth-resolved imaging by combining optical coherence tomography with pulsed laser excitation. This new method provides non-contact optical absorption contrast, complementing existing scattering-based imaging techniques.

    Area of Science:

    • Biomedical Optics
    • Optical Imaging
    • Photoacoustics

    Background:

    • Photoacoustic remote sensing microscopy (PARS) offers high-sensitivity, non-contact optical absorption contrast in scattering media.
    • Current PARS methods achieve depth resolution solely through optical sectioning, limiting their capabilities.
    • Contact-based photoacoustic modalities offer depth resolution via acoustic signal collection, but require physical contact.

    Purpose of the Study:

    • To introduce a novel coherence-gated approach for depth-resolved PARS imaging.
    • To validate the proposed method using simulations that incorporate laser-induced effects.
    • To explore key parameters for optimizing image quality in the new PARS technique.

    Main Methods:

    • Developed a coherence-gated PARS technique by analyzing differences in optical coherence tomography (OCT) scan-lines acquired with and without pulsed laser excitation.

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  • Validated the method through simulations accounting for pulsed-laser induced initial pressures and refractive index changes.
  • Investigated the relationship between refractive index changes and optical absorption.

    • Main Results:

    • Demonstrated that refractive index changes are directly proportional to optical absorption.
    • Identified critical parameters, including interrogation pulse duration and delay, essential for optimal image quality.
    • Simulations confirmed the feasibility of the proposed coherence-gated PARS approach.

    Conclusions:

    • The novel coherence-gated PARS method enables depth-resolved optical absorption contrast at optical-resolution scales.
    • This technique offers non-contact imaging capabilities, overcoming limitations of traditional PARS.
    • The approach holds promise for complementing OCT's scattering contrast in biomedical imaging.