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Related Concept Videos

Flow Cytometry01:23

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Rapidly Varying Flow01:24

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Rapidly varying flow (RVF) in open channels is characterized by abrupt changes in flow depth over a short distance, with the rate of depth change relative to distance often approaching unity. These flows are inherently complex due to their transient and multi-dimensional nature, making exact analysis difficult. However, approximate solutions using simplified models provide valuable insights into their behavior.Key Features of Rapidly Varying FlowRVF is commonly observed in scenarios involving...
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Substitution Rule Applied to Indefinite Integrals01:27

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When a force is applied to a linear spring, the restoring force increases proportionally with the amount of displacement. This behavior is described by Hooke’s law, which allows the work done on the spring to be determined directly from the force–displacement relationship. In this case, the force varies in a simple and predictable manner, making the calculation relatively simple.On the other hand, a nonlinear spring does not obey Hooke’s law. Its restoring force depends on...
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Substitution Rule Applied to Definite Integrals01:24

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When evaluating a definite integral whose integrand matches the structure of a composite function, the substitution method provides an efficient way to simplify the calculation. This method is based on reversing the chain rule from differentiation, allowing a complicated expression to be rewritten in a simpler form. When the integrand contains an inner function and its derivative, substitution naturally reduces the complexity of the problem.The core idea of substitution for definite integrals...
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Gene Flow02:39

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RACE - Rapid Amplification of cDNA Ends02:35

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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
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Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence
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Case Study: Applying Rapid Flow Cytometry Analysis to CAPD Effluent.

Kieran T Mulroney1,2, Jarrad M Hall3, Amanda L McGuire1,2

  • 1Translational Renal Research Group, Harry Perkins Institute of Medical Research, Queen Elizabeth II Medical Centre, Nedlands, Western Australia, Australia.

Peritoneal Dialysis International : Journal of the International Society for Peritoneal Dialysis
|September 7, 2018
PubMed
Summary
This summary is machine-generated.

Rapid flow cytometry accurately counts bacteria and immune cells in peritoneal dialysis (PD) peritonitis effluent within two hours. This faster method aids timely clinical decisions for antibiotic treatment in PD patients.

Keywords:
Peritonitisculture-independent microbiologydiagnostics

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Area of Science:

  • Clinical microbiology
  • Flow cytometry applications
  • Peritoneal dialysis complications

Background:

  • Peritonitis is a serious complication of peritoneal dialysis (PD), necessitating prompt clinical intervention.
  • Current culture-dependent methods for diagnosing PD peritonitis are time-consuming, delaying crucial antimicrobial treatment.
  • Existing genotypic methods have shown limited efficacy in analyzing PD effluent, leaving many centers reliant on traditional cultures.

Observation:

  • This study explored the application of flow cytometry to analyze antibiotic-compromised peritoneal dialysis effluent.
  • The technique was utilized to directly enumerate bacterial and human immune cells present in the effluent specimens.

Findings:

  • Flow cytometry successfully provided direct enumeration of both bacterial and human immune cells.
  • Results from flow cytometry analysis were available within 2 hours of specimen receipt, significantly faster than conventional methods.

Implications:

  • This rapid diagnostic approach using flow cytometry can significantly shorten the time to results for PD peritonitis.
  • Faster identification of causative agents and host response can lead to more informed and timely antimicrobial prescription.
  • This technique offers a promising alternative to slow culture-based diagnostics for managing PD peritonitis.