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Related Concept Videos

What is Gene Expression?01:42

What is Gene Expression?

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Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
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A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
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The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
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Microarray data analysis to identify differentially expressed genes and biological pathways associated with asthma.

Shanshan Qi1, Guanghui Liu2, Xiang Dong2

  • 1Department of Allergy, Wuhan No. 1 Hospital, Wuhan, Hubei 430022, P.R. China.

Experimental and Therapeutic Medicine
|September 7, 2018
PubMed
Summary
This summary is machine-generated.

This study identified key genes and biological pathways involved in asthma. Differentially expressed genes related to immune response and oxidation reduction offer potential targets for asthma diagnosis and treatment.

Keywords:
asthmabiological processdifferentially expressed genesprotein-protein interaction network

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Area of Science:

  • Genomics
  • Immunology
  • Molecular Biology

Background:

  • Asthma is a chronic respiratory disease characterized by airway inflammation.
  • Identifying specific genes and pathways involved in asthma pathogenesis is crucial for developing effective treatments.

Purpose of the Study:

  • To identify differentially expressed genes (DEGs) in allergic asthma compared to healthy controls.
  • To elucidate the biological processes and pathways associated with asthma.
  • To validate key findings using an independent dataset.

Main Methods:

  • Differential gene expression analysis of asthma datasets (GSE15823, GSE41649).
  • Gene Ontology and KEGG pathway enrichment analyses.
  • Protein-protein interaction network construction and module analysis.

Main Results:

  • Identified 43 upregulated and 275 downregulated DEGs in asthma.
  • Upregulated DEGs (e.g., NOS2) linked to oxidation reduction pathways.
  • Downregulated DEGs (e.g., CCL21, CXCL9) associated with immune response pathways.
  • PTPRC, CCL21, and CXCL9 identified as hub genes.
  • Validation confirmed consistent gene expression patterns across datasets.

Conclusions:

  • Immune response and oxidants/nitric oxide metabolic pathways are critical in asthma progression.
  • DEGs such as PTPRC, CCL21, CXCL9, and NOS2 represent potential diagnostic and therapeutic targets for asthma.