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Andrographolide attenuates viral myocarditis through interactions with the IL-10/STAT3 and P13K/AKT/NF-κβ signaling
Yaxin Zhao1, Meng Wang2, Yang Li3
1Department of Pharmacy, Jining No. 1 People's Hospital, Jining, Shandong 272000, P.R. China.
Insights
Andrographolide effectively treats viral myocarditis (VMC) by reducing inflammation and improving cardiac function. This natural compound modulates key signaling pathways, offering a potential therapeutic strategy for VMC.
Area of Science:
- Cardiology
- Immunology
- Pharmacology
Background:
- Viral myocarditis (VMC) is a severe cardiac condition leading to heart failure.
- Andrographolide, known for its anti-inflammatory properties, is explored for VMC treatment.
Purpose of the Study:
- To investigate the therapeutic effects of andrographolide on viral myocarditis (VMC).
- To elucidate the underlying molecular mechanisms of andrographolide in VMC treatment.
Main Methods:
- A VMC mouse model was utilized.
- Physiological and echocardiographic assessments were performed.
- Serum inflammatory markers (TNF-α, hs-CRP, cTnl) and protein expression (IL-10, STAT3, NF-κβ, Iκβα, P13K, AKT) were analyzed.
Main Results:
- Andrographolide treatment significantly reduced VMC-induced cardiac dysfunction and inflammation.
- It decreased serum levels of TNF-α, hs-CRP, and cTnl.
- Andrographolide modulated IL-10/STAT3 and NF-κβ signaling pathways, including reduced NF-κβ activation and increased Iκβα expression.
Conclusions:
- Andrographolide demonstrates significant therapeutic potential against viral myocarditis (VMC).
- Its efficacy is linked to the modulation of IL-10/STAT3 and NF-κβ signaling pathways.
- Andrographolide represents a promising agent for inhibiting VMC progression.
Abstract:
As a cardiac disease caused by the inflammation of the heart muscle, viral myocarditis (VMC) causes dilated cardiomyopathy, congestive heart failure and even death. With anti-inflammatory activities, andrographolide has been used in the treatment of various human diseases. In the present study, therapeutic effects of andrographolide on VMC were investigated using a VMC mouse model. Measurement of physiological indexes and echocardiographic examination was performed to explore the effects of andrographolide on cardiac function in mice with VMC. Levels of TNF-α, hs-CRP and cTnl in serum were measured by enzyme-linked immunosorbent assay (ELISA). Effects of andrographolide on the expression of L-10, STAT3, NF-κβ p65 and NF-κβ p50 were investigated by western blot analysis. Results indicated that andrographolide treatment reduced serum levels of TNF-α, hs-CRP and cTnl, increased the expression levels of IL-10 and STAT3 and reduced the expression levels of NF-κβ p65 and NF-κβ p50 and the phosphorylation levels of phosphoinositide 3-kinase (P13K) and AKT in the heart tissues of mice with VMC. In addition, andrographolide also increased the expression level of Iκβα in heart tissue. Therefore, it was concluded that andrographolide may inhibit the progression of VMC by interacting with the IL-10/STAT3 and NF-κβ signaling pathways.
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