Aberrantly Expressed Genes and miRNAs in Slow Transit Constipation Based on RNA-Seq Analysis
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Updated: Feb 5, 2026

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
Published on: March 12, 2021
Shipeng Zhao1, Qiang Chen1, Xianwu Kang1
1Department of Second Anorectal Section, The Third Hospital of Hebei Medical University, Shijiazhuang 050051, Hebei, China.
Background:
This study aims to identify the key genes and miRNAs in slow transit constipation (STC).
Methods:
MRNA and miRNA expression profiling were obtained. Differentially expressed genes (DEGs) and miRNAs were identified followed by the regulatory network construction. Functional annotation analysis and protein-protein interaction (PPI) network were conducted. The electronic validation was performed.
Results:
Hsa-miR-2116-3p, hsa-miR-3622a-5p, hsa-miR-424-5p, and hsa-miR-1273-3p covered most DEGs. HLA-DRB1, HLA-DRB5, C3, and ICAM were significantly involved in staphylococcus aureus infection. The PPI network generated several hub proteins including ZBTB16, FBN1, CCNF, and CDK1. Electronic validation of HLA-DRB1, PTGDR, MKI67, BIRC5, CCNF, and CDK1 was consistent with the RNA-sequencing analysis.
Conclusion:
Our study might be helpful in understanding the pathology of STC at the molecular level.
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