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Related Concept Videos

Proteomics01:33

Proteomics

9.8K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

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Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
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Overview of Exosomes01:36

Overview of Exosomes

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Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
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Buffer Effectiveness02:19

Buffer Effectiveness

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Buffer solutions do not have an unlimited capacity to keep the pH relatively constant . Instead, the ability of a buffer solution to resist changes in pH relies on the presence of appreciable amounts of its conjugate weak acid-base pair. When enough strong acid or base is added to substantially lower the concentration of either member of the buffer pair, the buffering action within the solution is compromised.
The buffer capacity is the amount of acid or base that can be added to a given volume...
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Framing Effects03:26

Framing Effects

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Information is everywhere and its presentation—such as how and when items are presented—can impact our perceptions and decisions surrounding the info. This broad concept umbrellas framing effects—influences that occur due to the way information is framed in its appearance, whether it’s purely the order or the specific wording of a message. Let’s take a look at numerous ways in which two versions of something can objectively say the same thing, yet we respond in...
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Aggregates Classification01:29

Aggregates Classification

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Aggregate classification is generally based on its size, petrographic characteristics, weight, and source. Size classification ranges from coarse to fine aggregates, defined by the size of the particles. Coarse aggregates are particles that do not pass through ASTM sieve No. 4, and aggregates that pass through the sieve are fine aggregates.
Petrographic classification groups aggregates based on common mineralogical characteristics. Some of the common mineral groups found in aggregates are...
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Related Experiment Video

Updated: Feb 5, 2026

SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells
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SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells

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AGGREGATION BY LECTIN-METHODICAL APPROACH FOR EFFECTIVE ISOLATION OF EXOSOMES FROM CELL CULTURE SUPERNATANT FOR

T A Shtam, V S Burdakov, S B Landa

    Tsitologiia
    |September 7, 2018
    PubMed
    Summary

    Researchers developed a new lectin-based method to easily isolate exosomes, which are potential cancer biomarkers. This technique simplifies exosome purification for protein biomarker research, overcoming a key challenge in diagnostic assay development.

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    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Background:

    • Exosomes are small vesicles secreted by cells, found in various bodily fluids.
    • They contain specific proteins and microRNAs, making them potential biomarkers for diseases like cancer.
    • Current exosome isolation methods are often complex, time-consuming, and instrument-dependent, hindering diagnostic applications.

    Purpose of the Study:

    • To develop a simplified and efficient method for exosome isolation.
    • To establish a lectin-based aggregation technique for preparative exosome purification.
    • To facilitate exosome-based protein biomarker research by improving sample preparation.

    Main Methods:

    • A novel preparative technique utilizing lectin-induced exosome aggregation was developed.
    • The method focuses on isolating exosomes from cell culture media.
    • This approach aims to streamline the sample preparation process for biomarker analysis.

    Main Results:

    • A new, efficient method for isolating exosomes was successfully established.
    • The lectin-based aggregation technique offers a simpler alternative to existing isolation procedures.
    • This method is particularly suited for preparing samples for exosome protein biomarker research.

    Conclusions:

    • Lectin-based aggregation provides a viable and less cumbersome approach for exosome isolation.
    • This technique addresses a significant bottleneck in developing exosome-based diagnostic assays.
    • The method enhances the potential for exosome research, especially in protein biomarker discovery.