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Related Concept Videos

Thermometers and Temperature Scales01:22

Thermometers and Temperature Scales

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Any physical property that depends consistently and reproducibly on temperature can be used as the basis of a thermometer. For example, volume increases with temperature for most substances. This property is the basis for the common alcohol thermometer and the original mercury thermometers. Other properties used to measure temperature include electrical resistance, color, and the emission of infrared radiation.
As many physical properties depend on temperature, the variety of thermometers is...
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Gas Thermometers and the Kelvin Scale01:22

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The definition of temperature in terms of molecular motion suggests that there should be a lowest possible temperature, where the average kinetic energy of molecules is zero (or the minimum allowed by quantum mechanics). Experiments confirm the existence of such a temperature, called absolute zero. An absolute temperature scale is one whose zero point is absolute zero. Such scales are convenient in science because several physical quantities, such as the volume of an ideal gas, are directly...
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Cancers Originate from Somatic Mutations in a Single Cell

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Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
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Voltaic/Galvanic Cells02:47

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Spontaneous Chemical Reactions
Spontaneous redox reactions occur abundantly in nature. The chemical reaction occurring in a disposable AA battery powering our remote controls is one such example of a spontaneous redox reaction. Another example is the immersion of coiled copper wire into an aqueous silver nitrate solution. The reaction shows a gradual, visually impressive color change from colorless to bright blue and the formation of a grey precipitate on the copper wire. In this experiment,...
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Concentration Cells

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A concentration cell is a type of a  voltaic cell constructed by connecting two almost identical half-cells, both based on the same half-reaction and using the same electrode, differing only in the concentration of one redox species. A concentration cell's potential, therefore, is determined only by the concentration difference of the particular redox species.
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DNA Base Pairing02:27

DNA Base Pairing

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Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
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Evaluating Plasmonic Transport in Current-carrying Silver Nanowires
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Silver Nanowire-Based Fluorescence Thermometer for a Single Cell.

Congcong Bu1,2, Lixuan Mu1, Xingxing Cao1,2

  • 1Key Laboratory of Photochemical Conversion and Optoelectronic Materials , Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Beijing 100190 , China.

ACS Applied Materials & Interfaces
|September 7, 2018
PubMed
Summary

Researchers developed a novel fluorescence thermometer using silver nanowires (AgNWs) and DNA. This thermometer can detect temperature changes within single cells, offering a new tool for cellular research.

Keywords:
DNA stem−loopfluorescencesilver nanowiressingle cellthermometer

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Area of Science:

  • Nanotechnology
  • Biophysics
  • Molecular Biology

Background:

  • Accurate temperature monitoring is crucial for understanding cellular processes.
  • Existing methods for intracellular temperature measurement have limitations.

Purpose of the Study:

  • To develop a sensitive and reliable fluorescence thermometer for intracellular temperature measurements.
  • To utilize silver nanowires (AgNWs) and DNA nanotechnology for temperature sensing.

Main Methods:

  • Assembling Texas Red (TR)-marked thermal-sensitive DNA stem-loops on AgNW surfaces.
  • Utilizing the temperature-dependent structural changes of DNA stem-loops to modulate energy transfer.
  • Measuring fluorescence intensity changes correlated with temperature variations.
  • Employing laser confocal microscopy for single-cell temperature observation.

Main Results:

  • The AgNW-based fluorescence thermometer demonstrated sensitivity in the 30-40 °C range with a sensitivity of 2.6%/°C.
  • Successfully observed temperature changes within a single cell using the developed thermometer.
  • The fluorescence intensity of the thermometer was sensitively controlled by temperature-induced structural changes in the DNA stem-loops.

Conclusions:

  • A novel AgNW-based fluorescence thermometer capable of sensitive intracellular temperature measurement was successfully realized.
  • The thermometer's performance relies on the temperature-dependent energy transfer between Texas Red and AgNWs, modulated by DNA stem-loop conformation.
  • This technology provides a promising tool for real-time monitoring of temperature dynamics in living cells.