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Updated: Feb 5, 2026

Analysis of Oxidative Stress in Zebrafish Embryos
Published on: July 7, 2014
Experimental Woodsmoke Exposure During Exercise and Blood Oxidative Stress
Bridget Peters1, Christopher Ballmann, Tiffany Quindry
1School of Kinesiology, Auburn University, Auburn, Alabama (Dr Peters, Dr Ballmann, Ms Zehner, Mr McCroskey); Department of Health and Human Performance (Ms Quindry, Dr Dumke, Dr Quindry); Center for Environmental Health Sciences (Mr Ferguson); School of Public and Community Health Sciences (Dr Ward), University of Montana, Missoula, Montana.
Objectives:
The current laboratory study quantified blood oxidative stress to woodsmoke exposure.
Methods:
Participants inhaled woodsmoke during three randomized crossover exercise trials (Clean Air [0 μg/m], Low Exposure [250 μg/m], and High Exposure [500 μg/m], Woodsmoke [particulate matter less than 2.5 μm, PM2.5]). Trolox equivalent antioxidant capacity (TEAC), uric acid (UA), 8-isoprostanes (8-ISO), lipid hydroperoxides (LOOH), protein carbonyls (PC), nitrotyrosine (3-NT), 8-isoprostane, and myeloperoxidase (MPO) were quantified in Pre, immediately Post, and 1- (1Hr) hour post blood samples.
Results:
UA decreased following Low Exposure, while plasma TEAC levels increased Post and 1Hr. LOOH levels decreased 1Hr Post (High Exposure), while 8-Iso increased following both smoke trials. PC and MPO were unchanged following all trials, while 3-NT increased over Clean Air.
Conclusion:
Blood oxidative stress occurred largely independent of PM2.5 concentrations. Future studies should employ longer duration smoke and exercise combined with physiologic parameters.
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