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A Novel Fluorescent Biosensor for Adenosine Triphosphate Detection Based on a Metal⁻Organic Framework Coating
1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. peipeixu@yeah.net.
Materials (Basel, Switzerland)
|September 8, 2018
Summary
A new fluorescent biosensor using polydopamine coated Zr-based metal-organic framework (PDA/UiO-66) offers sensitive detection of adenosine triphosphate (ATP). This novel approach enhances detection limits for potential clinical applications.
Area of Science:
- Materials Science
- Nanotechnology
- Analytical Chemistry
Background:
- Adenosine triphosphate (ATP) is a crucial biomarker for various biological processes.
- Existing methods for ATP detection often lack sensitivity or require complex procedures.
- Developing sensitive and efficient biosensors for ATP is essential for diagnostics.
Purpose of the Study:
- To develop a novel and highly sensitive fluorescent biosensor for adenosine triphosphate (ATP) detection.
- To utilize polydopamine coated Zr-based metal-organic framework (PDA/UiO-66) nanoparticles as a core component of the biosensor.
- To investigate the enhanced performance of the PDA/UiO-66 based biosensor compared to previous methods.
Main Methods:
- Fabrication of polydopamine coated Zr-based metal-organic framework (PDA/UiO-66) nanoparticles.
- Design of a fluorescent assay utilizing FAM-labeled probes, DNase I, and aptamers.
- Demonstration of ATP-induced fluorescence quenching and signal recovery mechanism.
- Evaluation of biosensor sensitivity and limit of detection (LOD).
Main Results:
- The PDA/UiO-66 nanoparticles exhibited excellent fluorescence quenching properties.
- The developed biosensor achieved a sensitive detection limit of 35 nM for ATP.
- The PDA/UiO-66 based method showed a 5.7-fold improvement in LOD compared to UiO-66 alone.
- Enhanced biocompatibility and bioactivity were observed due to the polydopamine layer.
Conclusions:
- The novel PDA/UiO-66 based fluorescent biosensor provides a sensitive and efficient platform for ATP detection.
- The enhanced performance and biocompatibility suggest potential for in vivo small molecule detection.
- This strategy holds promise for future clinical diagnostic applications.
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