Related Experiment Video

Updated: Feb 5, 2026

Protective Efficacy and Pulmonary Immune Response Following Subcutaneous and Intranasal BCG Administration in Mice
06:32

Protective Efficacy and Pulmonary Immune Response Following Subcutaneous and Intranasal BCG Administration in Mice

Published on: September 19, 2016

11.1K

Mycobacterium indicus pranii protein MIP_05962 induces Th1 cell mediated immune response in mice

Ashish Sharma1, Mohd Saqib2, Javaid A Sheikh3

  • 1Kusuma School of Biological Science, Indian Institute of Technology, Hauz Khas, New Delhi, 110016, India.

International Journal of Medical Microbiology : IJMM
|September 8, 2018
PubMed

Insights

Mycobacterium indicus pranii (MIP) vaccine

Area of Science:

  • Immunology
  • Vaccinology
  • Mycobacterial research

Background:

  • The live Mycobacterium indicus pranii (MIP) vaccine shows promise against mycobacterial infections.
  • Identifying specific protective antigens within MIP is crucial for understanding its efficacy.

Purpose of the Study:

  • To investigate the antigenicity and immunogenicity of the MIP_05962 protein.
  • To assess its potential as a protective antigen in mycobacterial infections.

Main Methods:

  • The study examined the protein MIP_05962, homologous to HSP18 and antigen1 from M. leprae and M. tuberculosis, respectively.
  • Immunogenicity was assessed in mice, analyzing antibody responses, cellular immune modulation, cytokine production (IFN-γ, TNF-α, IL-2), and CD4+ effector memory.

Main Results:

  • MIP_05962 induced significant antibody production in immunized mice.
  • It modulated cellular immunity towards a protective Th1-type response.
  • Both CD4+ and CD8+ T cells produced key protective cytokines, and CD4+ effector memory was enhanced.

Conclusions:

  • MIP_05962 demonstrates significant antigenicity and immunogenicity.
  • It shows potential as a protective antigen contributing to MIP's vaccine efficacy.
  • Further research can leverage these findings for improved mycobacterial vaccines.
Keywords:
CD4(+)cellsCD8(+)cellsImmunomodulationMycobacterium indicus praniiSmall heat shock proteinsTh1 immune responseTuberculosis

More Related Videos

Separation and Fractionation of Cell Wall and Cell Membrane Proteins from Mycobacterium tuberculosis for Downstream Protein Analysis06:14

Separation and Fractionation of Cell Wall and Cell Membrane Proteins from Mycobacterium tuberculosis for Downstream Protein Analysis

Published on: September 26, 2025

678
An In Vivo Mouse Model to Measure Naïve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells08:39

An In Vivo Mouse Model to Measure Naïve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells

Published on: August 22, 2018

20.5K

Related Concept Videos

Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

Overview
84.1K
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
7.5K
Humoral Immune Responses01:36

Humoral Immune Responses

Overview
84.0K
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
8.9K
Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
9.3K
Immune Response Against Viral Pathogens01:29

Immune Response Against Viral Pathogens

The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
2.0K