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RNA-Binding Protein RBP-P Is Required for Glutelin and Prolamine mRNA Localization in Rice Endosperm Cells.

Li Tian1, Hong-Li Chou1,2, Laining Zhang1

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Rice RNA binding protein RBP-P is crucial for correctly localizing storage protein mRNAs in developing grains. Loss of RBP-P function causes varied developmental defects and impacts essential gene regulation.

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Area of Science:

  • Plant Molecular Biology
  • Molecular Genetics
  • Biochemistry

Background:

  • During rice endosperm development, major storage protein mRNAs (glutelin, prolamine) are localized to specific endoplasmic reticulum sites.
  • RNA binding protein RBP-P interacts with both glutelin and prolamine mRNAs, indicating a role in their RNA metabolism.

Purpose of the Study:

  • To investigate the function of rice RNA binding protein RBP-P in mRNA localization and plant development.
  • To elucidate the molecular mechanisms underlying RBP-P's role in storage protein gene regulation and grain development.

Main Methods:

  • Generation and analysis of rice lines expressing mutant RBP-P.
  • Assessment of mRNA localization and protein-protein interactions.
  • Phenotypic analysis and transcriptome profiling of mutant rice lines.

Main Results:

  • Mutant RBP-P impaired the localization of glutelin and prolamine mRNAs, correlating with reduced RNA binding and/or protein interaction.
  • Partial loss of RBP-P function resulted in diverse developmental abnormalities, including dwarfism, chlorophyll deficiency, sterility, late flowering, and reduced spikelet fertility.
  • Transcriptome analysis revealed RBP-P's essential role in regulating storage protein genes and other critical biological processes in grain development.

Conclusions:

  • Rice RBP-P is essential for the proper localization of glutelin and prolamine mRNAs.
  • RBP-P's RNA binding activity and cooperative interactions are vital for regulating genes involved in plant growth and development.
  • Dysfunctional RBP-P leads to significant developmental defects in rice, highlighting its broad regulatory role.