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Updated: Feb 5, 2026

Fabrication of Refractive-index-matched Devices for Biomedical Microfluidics
Published on: September 10, 2018
Intrinsic refractive index matched 3D dSTORM with two objectives: Comparison of detection techniques
Nora C Schmidt1,2, Martin Kahms2, Jana Hüve3,4
1Fluorescence Microscopy Facility Münster (FM)2, Institute of Medical Physics and Biophysics, Center For NanoTechnology (CeNTech), Heisenbergstraße 11, 48149, Münster, Germany.
Abstract:
We have built a setup for 3D single molecule localisation microscopy (SMLM) where a very high resolution is achieved by, firstly, the use of two objectives instead of one and, secondly, minimizing optical aberrations by refractive index matching with a glycerol-water mixture as immersion medium in conjunction with glycerol-immersion objectives. Multiple optical paths of the microscope allow to switch between astigmatic and interferometric localisation along the optical axis, thus enabling a direct comparison of the performance of these localisation methods.
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