Related Experiment Video

Updated: Feb 5, 2026

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
11:22

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Published on: August 26, 2018

9.3K

Alterations in the expression and activity of pre-mRNA splicing factors in hepatocarcinogenesis

Carmen Berasain1,2,1,2, María Elizalde1,2,1,2, Raquel Urtasun1,2,1,2

  • 1Division of Hepatology & Gene Therapy, Centro de Investigación Médica Aplicada (CIMA), Universidad de Navarra, CIMA, Avda, Pio XII, n55, 31008 Pamplona, Spain.

Hepatic Oncology
|September 8, 2018
PubMed

Insights

Altered pre-mRNA splicing, driven by changes in splicing factors, significantly contributes to hepatocellular carcinoma (HCC) development. Understanding these splicing dysregulations is crucial for developing new HCC therapies.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Hepatocellular carcinoma (HCC) is a deadly cancer resistant to current treatments.
  • Pre-mRNA splicing dysregulation is increasingly recognized as a key factor in HCC development.
  • Alternative splicing can produce tumor-promoting protein isoforms and unstable mRNA.

Purpose of the Study:

  • To review recent findings on altered splicing factors in HCC.
  • To discuss the pathological significance of these splicing alterations.
  • To identify signaling pathways involved in HCC-related gene missplicing.

Main Methods:

  • Review of recent scientific literature on splicing factors in HCC.
  • Analysis of next-generation sequencing data implicating splicing dysregulation.
  • Discussion of molecular mechanisms and signaling pathways.

Main Results:

  • Significant alterations in splicing factor expression are observed in HCC.
  • These changes contribute to the expression of protumorigenic isoforms.
  • Specific cell signaling pathways are linked to aberrant splicing in hepatocarcinogenesis.

Conclusions:

  • Splicing factor alterations are critical drivers of HCC.
  • Targeting splicing machinery presents a potential therapeutic strategy for HCC.
  • Further research into splicing factor roles can illuminate HCC pathogenesis.

More Related Videos

Using the E1A Minigene Tool to Study mRNA Splicing Changes10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.5K
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.4K

Related Concept Videos

Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

6.9K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
8.2K
pre-mRNA Processing02:01

pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
57.6K
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
25.2K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
15.2K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
6.7K