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Updated: Feb 5, 2026

Collection and Long-Term Maintenance of Leaf-Cutting Ants Atta in Laboratory Conditions
Published on: August 30, 2022
Ants Do Not Traverse the Silk of Adult Female Nephila clavipes (Linnaeus) Webs
1Entomology and Plant Pathology, Graduate Program in Environmental Science, Oklahoma State Univ, Stillwater, OK, USA. Elise.Knowlton@okstate.edu.
Abstract:
Many organisms use chemicals to deter enemies. Some spiders can modify the composition of their silk to deter predators from climbing onto their webs. The Malaysian golden orb-weaver Nephila antipodiana (Walckenaer) produces silk containing an alkaloid (2-pyrrolidinone) that functions as a defense against ant invasion-ants avoid silk containing this chemical. In the present study, we test the generality of ants' silk avoidance behavior in the field. We introduced three ant species to the orb webs of Nephila clavipes (Linnaeus) in the tropical rainforest of La Selva, Costa Rica. We found that predatory army ants (Eciton burchellii Westwood) as well as non-predatory leaf-cutting ants (Atta cephalotes Linnaeus and Acromyrmex volcanus Wheeler) avoided adult N. clavipes silk, suggesting that an additional species within genus Nephila may possess ant-deterring silk. Our field assay also suggests that silk avoidance behavior is found in multiple ant species.
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