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Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.8K

Screening circular RNA related to chemotherapeutic resistance in osteosarcoma by RNA sequencing

Zhu Kun-Peng1,2, Ma Xiao-Long1,2, Zhang Lei1,2

  • 1Department of Orthopedics, Shanghai Tenth People's Hospital, Tongji University, School of Medicine, Shanghai 200072, PR China.

Epigenomics
|September 8, 2018
PubMed
Abstract

Insights

Circular RNAs (circRNAs) are linked to osteosarcoma (OS) chemoresistance. The study identified hsa_circ_0004674 as a potential biomarker for predicting treatment outcomes in OS patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Osteosarcoma (OS) is a primary bone malignancy.
  • Chemoresistance remains a significant challenge in OS treatment.
  • Circular RNAs (circRNAs) are emerging as key regulators in various cancers.

Purpose of the Study:

  • To identify circRNAs associated with chemoresistance in osteosarcoma.
  • To investigate the potential role of specific circRNAs as biomarkers for OS chemoresistance.

Main Methods:

  • Next-generation sequencing to profile circRNA expression in chemoresistant and chemosensitive OS cell lines.
  • Quantitative real-time PCR (qRT-PCR) for validation.
  • Bioinformatics analysis to predict circRNA functions and pathways.

Main Results:

  • Eighty dysregulated circRNAs were identified in chemoresistant OS cells.
  • Bioinformatics analysis revealed enrichment of drug metabolism pathways.
  • Hsa_circ_0004674 was significantly upregulated in chemoresistant OS cells and tissues, correlating with poor prognosis.

Conclusions:

  • CircRNAs play a role in the development of osteosarcoma chemoresistance.
  • Hsa_circ_0004674 is a potential diagnostic and prognostic biomarker for OS chemoresistance.
Keywords:
chemoresistancecircRNAosteosarcoma

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