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Experimental Approaches to Tissue Engineering
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Bioinspired Multi-Activities 4D Printing Objects: A New Approach Toward Complex Tissue Engineering.

Chloé D Devillard1, Céline A Mandon1, Simon A Lambert2

  • 1Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, ICBMS UMR 5246 CNRS, Université de Lyon, Université Lyon 1, CNRS, INSA Lyon, CPE Lyon. 43 bd du 11 Novembre 1918, 69622, Villeurbanne Cedex, France.

Biotechnology Journal
|September 8, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces 4D printing to create smart materials with biological functions. The 4D printed objects can perform localized calcification and form cell-laden fibrin biofilms for tissue engineering.

Keywords:
3D printing4D printingenzyme printingtissue engineering

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • 4D Printing Technology

Background:

  • 4D printing offers a pathway to advanced smart materials with dynamic properties.
  • Developing 4D printed constructs with multiple, controllable biological activities remains a challenge.

Purpose of the Study:

  • To present a novel strategy for creating 4D printed objects with dual biological activities.
  • To demonstrate the utility of these constructs in in vitro tissue engineering applications.

Main Methods:

  • Incorporation of alkaline phosphatase and thrombin enzymes during the 3D printing process.
  • Characterization using enzymatic assays, microscopy, magnetic resonance imaging (MRI), and cell seeding.
  • Evaluation of localized calcification and fibrin biofilm formation.

Main Results:

  • Successfully entrapped enzymes within the 4D printed hydrogel matrix.
  • Demonstrated pre-programmed, localized calcification of specific object regions.
  • Showcased thrombin-mediated fibrin biofilm formation with viable cells on the object surface.

Conclusions:

  • The developed 4D printing strategy enables the creation of multifunctional constructs for tissue engineering.
  • The localized biological activities are controllable and useful for in vitro applications.
  • This approach advances the potential of 4D printing for creating complex biological materials.