Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

28.1K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.1K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

5.6K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
5.6K
Embryonic Stem Cells00:58

Embryonic Stem Cells

32.5K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.5K
Embryonic Stem Cells00:57

Embryonic Stem Cells

5.1K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
5.1K
Adult Stem Cells01:33

Adult Stem Cells

33.9K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.9K
iPS Cell Differentiation01:22

iPS Cell Differentiation

3.1K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preclinical immunogenicity and safety evaluation of MDCK cell-derived quadrivalent influenza virus subunit vaccine.

Frontiers in immunology·2026
Same author

Modeling immune responses to autologous and allogeneic human stem cell-derived islet grafts in vivo.

JCI insight·2026
Same author

Asymmetric attrition and secondary chromosome destabilization after double-strand breaks in human embryonic development.

Nature communications·2026
Same author

A loss of function variant in <i>SLC30A8/ZnT8</i> drives proteomic changes associated with lowered apoptosis in human stem cell-derived islets.

medRxiv : the preprint server for health sciences·2026
Same author

Dual-anion NiSSe electrocatalyst enabling sulfur-tolerant sulfion oxidation for energy-efficient hydrogen production.

Journal of colloid and interface science·2026
Same author

Cellular signatures of melanocortin pathway genes across the locus coeruleus.

Acta neuropathologica communications·2026

Related Experiment Video

Updated: Feb 5, 2026

Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System
10:12

Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System

Published on: December 16, 2021

3.2K

Pancreatic Beta Cell Differentiation From Human Pluripotent Stem Cells.

Lina Sui1, Rudolph L Leibel1, Dieter Egli1

  • 1Naomi Berrie Diabetes Center & Department of Pediatrics, College of Physicians and Surgeons, Columbia University Medical Center, New York.

Current Protocols in Human Genetics
|September 8, 2018
PubMed
Summary

Researchers developed a new method to generate insulin-producing beta cells from human pluripotent stem cells (hPSCs). This technique aids diabetes research by providing a more accessible source of beta cells for study.

Keywords:
differentiationhuman pluripotent stem cellspancreatic beta cells

More Related Videos

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors
09:23

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors

Published on: March 7, 2017

8.4K
Author Spotlight: Advancements and Challenges in &#946;-Cells Differentiation from Pluripotent Stem Cells
06:33

Author Spotlight: Advancements and Challenges in β-Cells Differentiation from Pluripotent Stem Cells

Published on: February 2, 2024

2.6K

Related Experiment Videos

Last Updated: Feb 5, 2026

Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System
10:12

Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System

Published on: December 16, 2021

3.2K
Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors
09:23

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors

Published on: March 7, 2017

8.4K
Author Spotlight: Advancements and Challenges in &#946;-Cells Differentiation from Pluripotent Stem Cells
06:33

Author Spotlight: Advancements and Challenges in β-Cells Differentiation from Pluripotent Stem Cells

Published on: February 2, 2024

2.6K

Area of Science:

  • Endocrinology
  • Stem Cell Biology
  • Diabetes Research

Background:

  • Human beta cells are essential for glucose homeostasis and diabetes research.
  • Limited access to human beta cells hinders understanding of diabetes pathogenesis.
  • Current culture systems struggle to maintain beta cell identity and function post-isolation.

Purpose of the Study:

  • To develop a technique for generating functional beta cells from human pluripotent stem cells (hPSCs).
  • To overcome limitations in accessing and culturing human beta cells for research.
  • To investigate the potential of directed differentiation for beta cell generation.

Main Methods:

  • Directed differentiation of hPSCs into endodermal anlagen.
  • Modification of key signaling pathways using specific growth factors and small molecules.
  • 27-day differentiation protocol to promote beta cell development.

Main Results:

  • Generation of up to 70% C-peptide-positive beta cells.
  • 50% of differentiated cells were monohormonal, exhibiting characteristics of mature human beta cells.
  • A sub-population of cells co-expressed other endocrine markers, indicating immaturity.

Conclusions:

  • The described technique efficiently generates C-peptide-positive cells from hPSCs.
  • The generated cells show promise as a model for studying human beta cells.
  • Further refinement is needed to achieve full maturation and homogeneity of beta cells.