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Related Experiment Videos

Neutral proteases in human osteoarthritic synovium.

J Martel-Pelletier, J M Cloutier, J P Pelletier

    Arthritis and Rheumatism
    |September 1, 1986
    PubMed
    Summary

    Osteoarthritis (OA) synovial membranes show increased collagenolytic enzymes (CE) and proteoglycan-degrading enzymes (PE). This heightened enzyme activity, particularly serine proteases, suggests a role in OA joint tissue destruction and potential cartilage degradation.

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    Area of Science:

    • Biochemistry
    • Rheumatology
    • Molecular Biology

    Background:

    • Osteoarthritis (OA) is a degenerative joint disease characterized by cartilage breakdown.
    • Synovial inflammation plays a critical role in OA pathogenesis.
    • The role of specific enzymes in OA-related tissue destruction requires further elucidation.

    Purpose of the Study:

    • To quantify neutral collagenolytic enzyme (CE) and proteoglycan-degrading enzyme (PE) activities in OA synovial membranes.
    • To investigate the correlation between enzyme activity levels and the degree of synovial inflammation and hypertrophy in OA.
    • To assess the impact of steroid treatment on enzyme activity in OA synovium.

    Main Methods:

    • Analysis of neutral metallo-collagenolytic enzymes (CE) and proteoglycan-degrading enzymes (PE) activity in synovial membranes from OA patients and controls.

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  • Histological grading of synovial inflammation (mild, moderate, severe) and assessment of synovial hypertrophy.
  • Measurement of active and total enzyme forms, including serine proteases.
  • Evaluation of enzyme activity following steroid treatment.

    • Main Results:

    • Significantly elevated total neutral metallo-CE activity in OA synovia compared to controls.
    • Higher enzyme activity in severely and moderately inflamed synovia.
    • Steroid treatment reduced total metallo-CE activity.
    • Elevated active neutral metallo-CE and serine-CE activity in OA synovia, especially with severe inflammation and hypertrophy.
    • Significantly increased total and active neutral metallo-PE and serine-PE activity in OA synovia, particularly in severe inflammation.
    • Sustained high enzyme activity in OA synovium irrespective of hypertrophy or histological grade.

    Conclusions:

    • Increased neutral protease levels in OA synovium contribute to the local destruction of periarticular structures.
    • High levels of serine proteases may diffuse and degrade articular cartilage surface.
    • These findings highlight the enzymatic basis of tissue damage in osteoarthritis.