Related Experiment Video
Updated: Feb 5, 2026

Targeted DNA Methylation Analysis by Next-generation Sequencing
Published on: February 24, 2015
Pyrosequencing Analysis of MGMT Promoter Methylation in Meningioma
Ioannis Panagopoulos1, Ludmila Gorunova2, Henning Leske3
1Section for Cancer Cytogenetics, Institute for Cancer Genetics and Informatics, The Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway ioannis.panagopoulos@rr-research.no.
Background:
Methylation of the O6-methylguanine-DNA methyltransferase (MGMT) gene promoter is a well-established predictor of response to the DNA-alkylating agent temozolomide in patients with glioblastoma.
Materials And Methods:
Pyrosequencing analysis was used to determine the MGMT promoter methylation status in 61 meningiomas, to clarify whether it might have a predictive role.
Results:
Only two tumors (3%) had a mean methylation frequency higher than the cut-off value of 10% for the four CpG sites examined.
Conclusion:
The methylation of the MGMT promoter is uncommon, or occurs at a low frequency in meningiomas. There is no convincing rationale to test such tumors for their MGMT methylation status in a clinical setting.
More Related Videos
Related Concept Videos
The Eukaryotic Promoter Region
The Eukaryotic Promoter Region
Anaphase Promoting Complex
Models of Health Promotion and Illness Prevention II
The agent-host-environment model states that disease results...
Phase II Reactions: Methylation Reactions
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...
Levels of Health Promotion and Illness Prevention
In primary prevention, actions taken before disease onset prevent the disease from...

