Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Lung Capacity01:47

Lung Capacity

56.4K
The air in the lungs is measured in volumes and capacities. Lung volume measures reflect the amount of air taken in, released, or left over after a lung function, like a single inhalation. Lung capacity measures are sums of two or more lung volume measures.
56.4K
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

8.8K
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
8.8K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

7.4K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
7.4K
Pleura of the Lungs01:13

Pleura of the Lungs

7.4K
The lungs are nestled in a cavity, shielded by the pleura. The pleura, a form of serous membrane, wraps around each lung. This membrane arrangement consists of two layers: the visceral and parietal pleurae. The visceral pleura lines the surface of the lungIn contrast, the parietal pleura is the outer layer and contacts to the thoracic wall, the mediastinum, and the diaphragm. The hilum is the point of connection between the visceral and parietal layers. The space between the parietal and...
7.4K
T Cell Types and Functions01:24

T Cell Types and Functions

2.5K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
2.5K
Gross Anatomy of the Lungs01:17

Gross Anatomy of the Lungs

4.8K
The lungs are a pair of vital organs connected to the trachea via the left and right bronchi. The base of these organs meets the dome-shaped muscle known as the diaphragm. Encased by the pleurae, the lungs contact the mediastinum. The right lung is shorter yet wider, and has a larger volume than the left lung. The left lung has an indentation known as the cardiac notch. The superior region of the lungs is referred to as the apex, whereas the base is the lower region near the diaphragm. The...
4.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multiscale metabolic mapping of lung tissue via coregistered mass spectrometry and nonlinear optical imaging.

Science advances·2026
Same author

Editorial for Special Issue "Biemann: Embracing the Unknown".

Journal of the American Society for Mass Spectrometry·2026
Same author

Mood Altering Waters: Multidimensional Profiling and Chiral Characterization of Antidepressants in Effluent-Impacted Waterways.

Environmental science & technology·2026
Same author

PFOA induced metabolic and immune perturbations in a SARS-2 infection model.

bioRxiv : the preprint server for biology·2026
Same author

Small partial deletion of a highly GC-rich FOXF1 exon 1 in two deceased siblings with alveolar capillary dysplasia.

Genomics·2026
Same author

Characterization of Genetic Etiologic Factors for Pediatric Acute Lymphoblastic Leukemia in Large Childhood Cancer Survivorship Cohorts.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2026

Related Experiment Video

Updated: Feb 5, 2026

Author Spotlight: Enhancing Diagnostic Strategies and Biomarker Development for Comprehensive Lung Function Analysis
05:56

Author Spotlight: Enhancing Diagnostic Strategies and Biomarker Development for Comprehensive Lung Function Analysis

Published on: August 9, 2024

2.5K

Cell type-resolved human lung lipidome reveals cellular cooperation in lung function.

Jennifer E Kyle1, Geremy Clair1, Gautam Bandyopadhyay2

  • 1Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, 99352, USA.

Scientific Reports
|September 9, 2018
PubMed
Summary

This study maps the human lung lipidome for the first time, revealing significant cell-specific lipid variations. These findings highlight cellular cooperation in lung function.

More Related Videos

Three-Dimensional Phase Resolved Functional Lung Magnetic Resonance Imaging
10:44

Three-Dimensional Phase Resolved Functional Lung Magnetic Resonance Imaging

Published on: June 21, 2024

1.2K
Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture
11:49

Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture

Published on: July 21, 2020

9.1K

Related Experiment Videos

Last Updated: Feb 5, 2026

Author Spotlight: Enhancing Diagnostic Strategies and Biomarker Development for Comprehensive Lung Function Analysis
05:56

Author Spotlight: Enhancing Diagnostic Strategies and Biomarker Development for Comprehensive Lung Function Analysis

Published on: August 9, 2024

2.5K
Three-Dimensional Phase Resolved Functional Lung Magnetic Resonance Imaging
10:44

Three-Dimensional Phase Resolved Functional Lung Magnetic Resonance Imaging

Published on: June 21, 2024

1.2K
Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture
11:49

Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture

Published on: July 21, 2020

9.1K

Area of Science:

  • Pulmonary Biology
  • Lipidomics
  • Proteomics

Background:

  • Previous cell type-resolved proteome analyses exist for brain, heart, and liver.
  • A similar comprehensive lipidome analysis for any organ, particularly the lung, is currently lacking.

Purpose of the Study:

  • To create the first cell type-resolved lipidome map of the human lung.
  • To characterize lipidomic variations across major human lung cell types.
  • To integrate lipidomics with proteomics for a comprehensive understanding of lung cell function.

Main Methods:

  • Applied liquid chromatography-tandem mass spectrometry (LC-MS/MS) for lipidome characterization.
  • Isolated major human lung cell types from donor samples.
  • Performed complementary cell type-resolved proteomics to confirm cell identity and function.

Main Results:

  • Generated the first comprehensive lipidome map of the human lung at a cell type-resolved level.
  • Identified significant variations in lipid composition across different lung cell types, particularly at the subclass and fatty acid chain length levels.
  • Demonstrated extensive cellular cooperation within the lung through integrated lipidomic and proteomic signatures.

Conclusions:

  • The human lung exhibits distinct cell type-specific lipid profiles.
  • Integrated lipidomic and proteomic data provide a valuable resource for understanding lung physiology and disease.
  • This study establishes a foundation for future research into lung lipid metabolism and function.